Genomic RNA profiling and the programme controlling preimplantation mammalian development.
Genomic RNA profiling and the programme controlling preimplantation mammalian development.
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DOI：
10.1093/molehr/gan063
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发表时间：
2008-12
影响因子：
4
通讯作者：
Watson, Andrew J.
中科院分区：
文献类型：
--
作者：
Bell, Christine E.;Calder, Michele D.;Watson, Andrew J.
着床前发育在受精后迅速从母体程序转变为胚胎程序。虽然大多数卵子发生产物在母体向胚胎过渡（MET）期间丢失，但有一些确实在此期间存活下来，直接有助于支持植入前发育。胚胎基因组激活（EGA）的特征在于MET所必需的几个基因的瞬时表达，虽然EGA代表了基因表达的第一个主要波，但已经发现了支持发育到胚泡阶段的第二个中期植入前转录波。基因组学方法的应用极大地帮助了阶段特异性基因表达模式的发现，现在的挑战是在很大程度上定义植入前发育过程中的基因功能和调控。控制压实，谱系规范和囊胚形成的基本机制被定义。对胚胎培养的要求揭示了发育程序的可塑性，这种可塑性可能超过胚胎的适应能力，并促进了旨在减轻培养引起的胚胎编程变化的重要研究方向。新的调控水平正在出现，需要对RNA结合蛋白和miRNA所起的作用有更深入的了解。所有这些研究都是相关的，因为有必要为胚胎移植生产健康的植入前胚胎，以确保以最有效和最安全的方式应用辅助生殖技术。
Preimplantation development shifts from a maternal to embryonic programme rapidly after fertilization. Although the majority of oogenetic products are lost during the maternal to embryonic transition (MET), several do survive this interval to contribute directly to supporting preimplantation development. Embryonic genome activation (EGA) is characterized by the transient expression of several genes that are necessary for MET, and while EGA represents the first major wave of gene expression, a second mid-preimplantation wave of transcription that supports development to the blastocyst stage has been discovered. The application of genomic approaches has greatly assisted in the discovery of stage specific gene expression patterns and the challenge now is to largely define gene function and regulation during preimplantation development. The basic mechanisms controlling compaction, lineage specification and blastocyst formation are defined. The requirement for embryo culture has revealed plasticity in the developmental programme that may exceed the adaptive capacity of the embryo and has fostered important research directions aimed at alleviating culture-induced changes in embryonic programming. New levels of regulation are emerging and greater insight into the roles played by RNA-binding proteins and miRNAs is required. All of this research is relevant due to the necessity to produce healthy preimplantation embryos for embryo transfer, to ensure that assisted reproductive technologies are applied in the most efficient and safest way possible.
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DOI：
10.1016/j.bbrc.2006.05.093
发表时间：
2006-07-21
影响因子：
3.1
作者：
Chen, Yu-Ling;Huang, Ya-Lin;Chang, Ching-Jin
通讯作者：
Chang, Ching-Jin
影响因子：
2.5
作者：
Alizadeh, Z;Kageyama, S;Aoki, F
通讯作者：
Aoki, F
影响因子：
2.7
作者：
BACHVAROVA, R;DELEON, V
通讯作者：
DELEON, V
影响因子：
1.9
作者：
Bettegowda, Anilkumar;Lee, Kyung-Bon;Smith, George W.
通讯作者：
Smith, George W.
影响因子：
2.5
作者：
Fair, T;Murphy, M;Lonergan, P
通讯作者：
Lonergan, P