Assembly of RNP granules in stressed and aging oocytes requires nucleoporins and is coordinated with nuclear membrane blebbing.
Assembly of RNP granules in stressed and aging oocytes requires nucleoporins and is coordinated with nuclear membrane blebbing.
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DOI：
10.1016/j.ydbio.2011.02.028
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发表时间：
2011-05-15
影响因子：
2.7
通讯作者：
Schisa JA
中科院分区：
文献类型：
--
作者：
Patterson JR;Wood MP;Schisa JA
生殖细胞对压力和衰老的保护性细胞反应对于物种的永存至关重要；然而，很少有研究关注生殖细胞如何应对压力和衰老。我们之前已经证明，在延长减数分裂停滞期间或环境应激后，大核糖核蛋白（RNP）复合物在秀丽隐杆线虫卵母细胞中组装。在这里，我们探索这些动态 RNP 的调节，并证明它们的组装与卵母细胞中戏剧性的核膜起泡相协调。我们的超微结构分析揭示了内质网的明显变化，以及秀丽隐杆线虫中堆叠的环状片层组装的第一个证据。我们进一步表明，RNP 颗粒的完整组装需要几种核孔蛋白，RNP 颗粒组装的破坏加上停滞卵母细胞中低频率的核起泡会对胚胎活力产生负面影响。我们的观察结果支持这样一种模型：在应激或减数分裂停滞的细胞中，需要核膜起泡来增加核孔蛋白向细胞皮层的运输，并促进 RNP 的 RNA 和蛋白质成分招募到大型复合物中。这些新见解可能对更好地理解生殖细胞在受精延迟时如何保持完整性以及细胞如何应对压力具有普遍意义。
Protective cellular responses to stress and aging in the germ line are essential for perpetuation of a species; however, relatively few studies have focused on how germ cells respond to stress and aging. We have previously shown that large ribonucleoprotein (RNP) complexes assemble in oocytes of Caenorhabditis during extended meiotic arrest or after environmental stress. Here we explore the regulation of these dynamic RNPs and demonstrate their assembly is coordinated with dramatic, nuclear membrane blebbing in oocytes. Our ultrastructural analyses reveal distinct changes in the endoplasmic reticulum, and the first evidence for the assembly of stacked annulate lamellae in Caenorhabditis. We further show several nucleoporins are required for the complete assembly of RNP granules, and a disruption in RNP granule assembly coupled with a low frequency of nuclear blebbing in arrested oocytes negatively impacts embryonic viability. Our observations support a model where nuclear membrane blebbing is required to increase the trafficking of nucleoporins to the cell cortex in stressed or meiotically-arrested cells and to facilitate the recruitment of RNA and protein components of RNPs into large complexes. These new insights may have general implications for better understanding how germ cells preserve their integrity when fertilization is delayed and how cells respond to stress.
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影响因子：
2.4
作者：
Jud, Molly;Razelun, Jamie;Schisa, Jennifer A.
通讯作者：
Schisa, Jennifer A.
影响因子：
64.5
作者：
Blower, MD;Nachury, M;Weis, K
通讯作者：
Weis, K
DOI：
10.1016/s0022-5320(71)80146-6
发表时间：
1971-01-01
期刊：
JOURNAL OF ULTRASTRUCTURE RESEARCH
影响因子：
--
作者：
GULYAS, BJ
通讯作者：
GULYAS, BJ
影响因子：
9.2
作者：
Cheng, Hua;Govindan, J. Amaranath;Greenstein, David
通讯作者：
Greenstein, David
影响因子：
3.5
作者：
Joseph, Jomon;Dasso, Mary
通讯作者：
Dasso, Mary