Development of marker-free transgenic Jatropha plants with increased levels of seed oleic acid.
Development of marker-free transgenic Jatropha plants with increased levels of seed oleic acid.
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DOI：
10.1186/1754-6834-5-10
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发表时间：
2012-02-29
影响因子：
6.3
通讯作者：
Ye J
中科院分区：
文献类型：
--
作者：
Qu J;Mao HZ;Chen W;Gao SQ;Bai YN;Sun YW;Geng YF;Ye J
麻风树被认为是一种新的能源作物，因为它的种子中含有大量的油，可以转化为生物柴油。生物柴油的质量和性能取决于油中脂肪酸的化学组成。油的脂肪酸组成对点火质量、燃烧热和氧化稳定性有直接影响。理想的生物柴油组合物应具有更多的单不饱和脂肪酸和更少的多不饱和酸。麻风树籽油含有30%至50%的多不饱和脂肪酸（主要是亚油酸），这对氧化稳定性产生负面影响，并导致氮氧化物的高排放率。1-酰基-2-油酰基-sn-甘油-3-磷酸胆碱δ 12-去饱和酶（FAD 2）是植物中负责亚油酸产生的关键酶。我们通过全基因组分析确定了麻风树中三个假定的δ 12脂肪酸去饱和酶基因（JcFAD 2s），并通过RNA干扰技术以种子特异性方式下调了其中一个基因JcFAD 2 -1的表达。得到的JcFAD 2 -1 RNA干扰转基因植物显示其种子油中油酸的显著增加（> 78%）和多不饱和脂肪酸的相应减少（< 3%）。对照麻风树含有约37%的油酸和41%的多不饱和脂肪酸。这表明FAD 2 -1是麻风树中负责将油酸转化为亚油酸的主要酶。由于脂肪酸谱的变化，估计JcFAD 2 -1 RNA干扰种子的油产生高达60.2的十六烷值，这与欧洲常规优质柴油燃料所需的十六烷值（60）相似。根据我们对温室条件下原始植物的初步数据，高种子油酸的存在对其他麻风树农艺性状没有负面影响。此外，我们开发了一种无标记系统来产生转基因麻风树，这将有助于减少公众对转基因植物周围环境问题的担忧。在这项研究中，我们产生了种子特异性JcFAD 2 -1 RNA干扰转基因麻风树没有选择标记。我们通过基因工程成功地提高了麻风树中油酸与亚油酸的比例，提高了其油的质量。
Jatropha curcas is recognized as a new energy crop due to the presence of the high amount of oil in its seeds that can be converted into biodiesel. The quality and performance of the biodiesel depends on the chemical composition of the fatty acids present in the oil. The fatty acids profile of the oil has a direct impact on ignition quality, heat of combustion and oxidative stability. An ideal biodiesel composition should have more monounsaturated fatty acids and less polyunsaturated acids. Jatropha seed oil contains 30% to 50% polyunsaturated fatty acids (mainly linoleic acid) which negatively impacts the oxidative stability and causes high rate of nitrogen oxides emission. The enzyme 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine delta 12-desaturase (FAD2) is the key enzyme responsible for the production of linoleic acid in plants. We identified three putative delta 12 fatty acid desaturase genes in Jatropha (JcFAD2s) through genome-wide analysis and downregulated the expression of one of these genes, JcFAD2-1, in a seed-specific manner by RNA interference technology. The resulting JcFAD2-1 RNA interference transgenic plants showed a dramatic increase of oleic acid (> 78%) and a corresponding reduction in polyunsaturated fatty acids (< 3%) in its seed oil. The control Jatropha had around 37% oleic acid and 41% polyunsaturated fatty acids. This indicates that FAD2-1 is the major enzyme responsible for converting oleic acid to linoleic acid in Jatropha. Due to the changes in the fatty acids profile, the oil of the JcFAD2-1 RNA interference seed was estimated to yield a cetane number as high as 60.2, which is similar to the required cetane number for conventional premium diesel fuels (60) in Europe. The presence of high seed oleic acid did not have a negative impact on other Jatropha agronomic traits based on our preliminary data of the original plants under greenhouse conditions. Further, we developed a marker-free system to generate the transgenic Jatropha that will help reduce public concerns for environmental issues surrounding genetically modified plants. In this study we produced seed-specific JcFAD2-1 RNA interference transgenic Jatropha without a selectable marker. We successfully increased the proportion of oleic acid versus linoleic in Jatropha through genetic engineering, enhancing the quality of its oil.
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影响因子：
5.3
作者：
Yi C;Zhang S;Liu X;Bui HT;Hong Y
通讯作者：
Hong Y
影响因子：
13.8
作者：
Graef, George;LaVallee, Bradley J.;Clemente, Tom E.
通讯作者：
Clemente, Tom E.
影响因子：
2.3
作者：
Bachlava, Eleni;Burton, Joseph W.;Cardinal, Andrea J.
通讯作者：
Cardinal, Andrea J.
影响因子：
11.6
作者：
JONES, A;DAVIES, HM;VOELKER, TA
通讯作者：
VOELKER, TA
影响因子：
2.3
作者：
Scherder, Curtis W.;Fehr, Walter R.
通讯作者：
Fehr, Walter R.