Comparative metabolomics profiling of engineered Saccharomyces cerevisiae lead to a strategy that improving β-carotene production by acetate supplementation.
Comparative metabolomics profiling of engineered Saccharomyces cerevisiae lead to a strategy that improving β-carotene production by acetate supplementation.
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工程酿酒酵母的比较代谢组学分析得出了通过补充醋酸盐提高β-胡萝卜素产量的策略
DOI：
10.1371/journal.pone.0188385
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Yan G
中科院分区：
文献类型：
--
作者：
Bu X;Sun L;Shang F;Yan G
采用气相色谱-质谱法(GC-MS)、高效液相色谱-质谱法(HPLC-MS)和超高效液相色谱-串联质谱法(UPLC-MS/MS)对产β-胡萝卜素的重组酿酒酵母菌株和以葡萄糖为碳源培养的亲本菌株进行比较代谢组学分析。结果表明，与亲本菌株相比，重组菌株中与氨基酸、碳水化合物、糖酵解和TCA循环中间体（乙酸、甘油、柠檬酸、丙酮酸和琥珀酸）、脂肪酸、麦角甾醇和能量代谢物相关的大多数中心中间体的产生量较低。为了提高重组菌株的β-胡萝卜素产量，开发了一种在指数生长期外源添加乙酸盐（10 g/l）的策略，该策略可以提高大多数细胞内代谢水平，使β-胡萝卜素浓度和产量分别提高39.3%和14.2%，同时细胞内乙酰辅酶A、脂肪酸、麦角甾醇和ATP含量增加。这些结果表明，工程菌株中细胞内代谢物的量很大程度上被类胡萝卜素的形成所消耗。因此，维持细胞内代谢物池在正常水平对于类胡萝卜素生物合成至关重要。为了缓解这一限制，合理补充乙酸盐可能是一种潜在的方法，因为它可以部分恢复重组酿酒酵母细胞内代谢物的水平并提高类胡萝卜素化合物的产量。
A comparative metabolomic analysis was conducted on recombinant Saccharomyces cerevisiae strain producing β-carotene and the parent strain cultivated with glucose as carbon source using gas chromatography-mass spectrometry (GC-MS), high performance liquid chromatography-mass spectrometry (HPLC-MS) and ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) based approach. The results showed that most of the central intermediates associated with amino acids, carbohydrates, glycolysis and TCA cycle intermediates (acetic acid, glycerol, citric acid, pyruvic acid and succinic acid), fatty acids, ergosterol and energy metabolites were produced in a lower amount in recombinant strain, as compared to the parent strain. To increase β-carotene production in recombinant strain, a strategy that exogenous addition of acetate (10 g/l) in exponential phase was developed, which could enhance most intracellular metabolites levels and result in 39.3% and 14.2% improvement of β-carotene concentration and production, respectively, which was accompanied by the enhancement of acetyl-CoA, fatty acids, ergosterol and ATP contents in cells. These results indicated that the amounts of intracellular metabolites in engineered strain are largely consumed by carotenoid formation. Therefore, maintaining intracellular metabolites pool at normal levels is essential for carotenoid biosynthesis. To relieve this limitation, rational supplementation of acetate could be a potential way because it can partially restore the levels of intracellular metabolites and improve the production of carotenoid compounds in recombinant S. cerevisiae.
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基于代谢组学的代谢途径工程赋予乙糖酵母的重组二甲糖发酵菌株的乙酸和甲酸耐受性。
影响因子：
6.4
作者：
Hasunuma T;Sanda T;Yamada R;Yoshimura K;Ishii J;Kondo A
通讯作者：
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影响因子：
4.4
作者：
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通讯作者：
Yan, Guoliang
影响因子：
5
作者：
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通讯作者：
Nielsen, J
影响因子：
3.2
作者：
Bandara, Ajith;Fraser, Sarah;Stanley, Grant A.
通讯作者：
Stanley, Grant A.
影响因子：
4.4
作者：
Martinez-Moya P;Niehaus K;Alcaíno J;Baeza M;Cifuentes V
通讯作者：
Cifuentes V