Baculovirus-mediated gene delivery and RNAi applications.

Baculovirus-mediated gene delivery and RNAi applications.
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DOI:
10.3390/v7042099
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发表时间:
2015-04-22
期刊:
Viruses
影响因子:
--
通讯作者:
Ylä-Herttulala S
Ylä-Herttulala S
中科院分区:
其他
文献类型:
--
作者:
Makkonen KE;Airenne K;Ylä-Herttulala S

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杆状病毒在自然界中广泛存在,并且关于其安全性和生物学的大量数据是可用的。最近,这些多功能的昆虫特异性病毒已经证明了它们在各种生物技术应用中的有用性,包括蛋白质生产和基因转移。存在多个体外和体内研究,并支持其用作脊椎动物细胞中的基因递送载体。近年来,杆状病毒在RNA干扰领域的应用也显示出了巨大的潜力,杆状病毒的一些优点使其成为一种安全性高、嗜性广的RNA基因转移工具。
Baculoviruses are widely encountered in nature and a great deal of data is available about their safety and biology. Recently, these versatile, insect-specific viruses have demonstrated their usefulness in various biotechnological applications including protein production and gene transfer. Multiple in vitro and in vivo studies exist and support their use as gene delivery vehicles in vertebrate cells. Recently, baculoviruses have also demonstrated high potential in RNAi applications in which several advantages of the virus make it a promising tool for RNA gene transfer with high safety and wide tropism.
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