Rapid Detection of Urinary Tract Infection in 10 min by Tracking Multiple Phenotypic Features in a 30 s Large-Volume Scattering Video of Urine Microscopy.
Rapid Detection of Urinary Tract Infection in 10 min by Tracking Multiple Phenotypic Features in a 30 s Large-Volume Scattering Video of Urine Microscopy.
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DOI：
10.1021/acssensors.2c00788
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发表时间：
2022-08-26
期刊：
影响因子：
8.9
通讯作者：
Wang, Shaopeng
中科院分区：
文献类型：
--
作者：
Zhang, Fenni;Mo, Manni;Jiang, Jiapei;Zhou, Xinyu;McBride, Michelle;Yang, Yunze;Reilly, Kenta S.;Grys, Thomas E.;Haydel, Shelley E.;Tao, Nongjian;Wang, Shaopeng
关键词：
细菌感染疾病的快速护理点 (POC) 诊断提供了迅速启动抗菌治疗和减少非细菌感染过度使用/误用不必要抗生素的临床益处。我们在此介绍一种可在 10 分钟内快速检测细菌感染的即时护理兼容方法。我们使用低放大倍率 (1-2X) 的大体积溶液散射成像 (LVSi) 系统来可视化临床样本中的细菌，从而消除了基于培养的分离和富集的需要。我们在一段短视频中跟踪了单个细胞的多种内在表型特征。通过使用简单的机器学习算法对这些特征进行聚类，我们可以区分大肠杆菌和类似大小的聚苯乙烯珠，区分不同形状的细菌，以及区分大肠杆菌和尿液颗粒。我们应用该方法通过 30 秒的 LVSi 视频检测 104 例患者尿液样本中的尿路感染，结果与临床培养结果相比，准确率为 92.3%。该技术为在护理点进行快速细菌感染诊断提供了机会。一种与护理点兼容的 UTI 筛查方法，基于对多种内在细菌表型特征的大体积溶液散射成像 (LVSi) 跟踪，可在 10 分钟内直接对尿液样本进行筛查，以实现快速感染检测（称为 LVSi-RD）。
Rapid point-of-care (POC) diagnosis of bacterial infection diseases provides clinical benefits of prompt initiation of the antimicrobial therapy and reduction of the overuse/misuse of unnecessary antibiotics for nonbacterial infections. We present here a point-of-care compatible method for rapid bacterial infection detection in 10 minutes. We use a large volume solution scattering imaging (LVSi) system with low magnifications (1–2X) to visualize bacteria in clinical samples, thus eliminating the need for culture-based isolation and enrichment. We tracked multiple intrinsic phenotypic features of individual cells in a short video. By clustering these features with a simple machine learning algorithm, we can differentiate Escherichia coli from similar-sized polystyrene beads, distinguish bacteria with different shapes, and distinguish E. coli from urine particles. We applied the method to detect urinary tract infections in 104 patient urine samples with a 30-second LVSi video, and the results showed 92.3% accuracy compared with the clinical culture results. This technology provides opportunities for rapid bacterial infection diagnosis at the point-of-care settings. A point-of-care compatible UTI screening approach performed directly on a urine sample in less than 10 minutes based on large volume solution scattering imaging (LVSi) tracking of multiple intrinsic bacterial phenotypic features for rapid infection detection (referred to as LVSi-RD).
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DOI：
10.1073/pnas.0607898104
发表时间：
2007-10-16
影响因子：
11.1
作者：
Lane, M. Chelsea;Alteri, Christopher J.;Mobley, Harry L. T.
通讯作者：
Mobley, Harry L. T.
影响因子：
3.8
作者：
Jo, YoungJu;Jung, JaeHwang;Park, YongKeun
通讯作者：
Park, YongKeun
DOI：
10.1038/nrurol.2017.20
发表时间：
2017-05
期刊：
Nature reviews. Urology
影响因子：
--
作者：
Davenport M;Mach KE;Shortliffe LMD;Banaei N;Wang TH;Liao JC
通讯作者：
Liao JC
影响因子：
4.6
作者：
Jo Y;Jung J;Lee JW;Shin D;Park H;Nam KT;Park JH;Park Y
通讯作者：
Park Y
影响因子：
6.6
作者：
Griebling, TL
通讯作者：
Griebling, TL