Angle-resolved light scattering of individual rod-shaped bacteria based on Fourier transform light scattering.
Angle-resolved light scattering of individual rod-shaped bacteria based on Fourier transform light scattering.
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DOI：
10.1038/srep05090
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发表时间：
2014-05-28
影响因子：
4.6
通讯作者：
Park Y
中科院分区：
文献类型：
--
作者：
Jo Y;Jung J;Lee JW;Shin D;Park H;Nam KT;Park JH;Park Y
在单细胞水平上测量单个杆状细菌的二维角度分辨光散射图。使用定量相位成像和傅里叶变换光散射技术，可以在-70°至70°的宽角度范围内以前所未有的灵敏度测量四种杆状物种（枯草芽孢杆菌、干酪乳杆菌、细长聚球藻和大肠杆菌）中单个细菌的光散射模式。沿着杆状细菌的两个主轴分析测量的光散射图案，以便系统地研究各向异性光散射的物种特异性特征。此外，计算并使用单个细菌的细胞干质量来证明细菌物种内光散射的细胞间变化与细胞干质量和生长相关。
Two-dimensional angle-resolved light scattering maps of individual rod-shaped bacteria are measured at the single-cell level. Using quantitative phase imaging and Fourier transform light scattering techniques, the light scattering patterns of individual bacteria in four rod-shaped species (Bacillus subtilis, Lactobacillus casei, Synechococcus elongatus, and Escherichia coli) are measured with unprecedented sensitivity in a broad angular range from −70° to 70°. The measured light scattering patterns are analyzed along the two principal axes of rod-shaped bacteria in order to systematically investigate the species-specific characteristics of anisotropic light scattering. In addition, the cellular dry mass of individual bacteria is calculated and used to demonstrate that the cell-to-cell variations in light scattering within bacterial species is related to the cellular dry mass and growth.
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影响因子：
48
作者：
Godin, Michel;Delgado, Francisco Feijo;Son, Sungmin;Grover, William H.;Bryan, Andrea K.;Tzur, Amit;Jorgensen, Paul;Payer, Kris;Grossman, Alan D.;Kirschner, Marc W.;Manalis, Scott R.
通讯作者：
Manalis, Scott R.
影响因子：
3.6
作者：
Auger, Jean-Claude;Aptowicz, Kevin B.;Chang, Richard K.
通讯作者：
Chang, Richard K.
影响因子：
3.8
作者：
Lee, KyeoReh;Kim, Hyeon-Don;Park, YongKeun
通讯作者：
Park, YongKeun
影响因子：
2
作者：
KOCH, AL
通讯作者：
KOCH, AL
影响因子：
12.6
作者：
Banada, Padmapriya P.;Huff, Karleigh;Bhunia, Arun K.
通讯作者：
Bhunia, Arun K.