Arsenic Accumulation in Hydroponically Grown Schizachyrium scoparium (Little Bluestem) Amended with Root-Colonizing Endophytes.
Arsenic Accumulation in Hydroponically Grown Schizachyrium scoparium (Little Bluestem) Amended with Root-Colonizing Endophytes.
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DOI：
10.1021/acsearthspacechem.0c00302
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发表时间：
2021-06-17
影响因子：
3.4
通讯作者：
Cerrato JM
中科院分区：
文献类型：
--
作者：
DeVore CL;Hayek EE;Busch T;Long B;Mann M;Rudgers JA;Ali AS;Howard T;Spilde MN;Brearley A;Ducheneaux C;Cerrato JM
我们综合了显微镜、光谱学、培养和分子生物学以及水化学技术来评估接种内生真菌的水培 Schizachyrium scoparium 中砷 (As) 的积累。 Schizachyrium scoparium 生长在夏延河流域历史上受污染的沉积物中，并用于在中性 pH 值下进行 As(V) 范围为 0 至 2.5 mg L−1 的实验室实验。几十年来，区域植物中砷的积累一直是社会关注的问题，但影响与内生真菌相关的植物中砷积累的机制仍然知之甚少。与未接种的植物相比，内生真菌在根部的定殖支持更好的外部和维管细胞结构、增加的生物量产量、增加的根长度和增加的磷吸收（p值<0.05）。暴露于 As(V) 后，溶液中的 As 减少了 80%，并且主要积累在未接种植物的根部 (0.82–13.44 mg kg−1)。内生真菌介导根细胞的细胞内摄取和砷的易位。电子探针X射线图谱分析在暴露植物的根部表面检测到含有As的Ca-P和Mg-P矿物质，表明这些矿物质可能通过表面络合或共沉淀导致As吸附在根部表面。我们的研究结果为影响植物中砷积累的生物和物理化学过程提供了新的见解，用于风险评估应用和生物修复策略。
We integrated microscopy, spectroscopy, culturing and molecular biology, and aqueous chemistry techniques to evaluate arsenic (As) accumulation in hydroponically grown Schizachyrium scoparium inoculated with endophytic fungi. Schizachyrium scoparium grows in historically contaminated sediment in the Cheyenne River Watershed and was used for laboratory experiments with As(V) ranging from 0 to 2.5 mg L−1 at circumneutral pH. Arsenic accumulation in regional plants has been a community concern for several decades, yet mechanisms affecting As accumulation in plants associated with endophytic fungi remain poorly understood. Colonization of roots by endophytic fungi supported better external and vascular cellular structure, increased biomass production, increased root lengths and increased P uptake, compared to noninoculated plants (p value <0.05). After exposure to As(V), an 80% decrease of As was detected in solution and accumulated mainly in the roots (0.82–13.44 mg kg−1) of noninoculated plants. Endophytic fungi mediated intracellular uptake into root cells and translocation of As. Electron microprobe X-ray mapping analyses detected Ca–P and Mg–P minerals with As on the root surface of exposed plants, suggesting that these minerals could lead to As adsorption on the root surface through surface complexation or coprecipitation. Our findings provide new insights regarding biological and physical–chemical processes affecting As accumulation in plants for risk assessment applications and bioremediation strategies.
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影响因子：
6.9
作者：
Batista BL;Nigar M;Mestrot A;Rocha BA;Barbosa Júnior F;Price AH;Raab A;Feldmann J
通讯作者：
Feldmann J
影响因子：
0.8
作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
2.5
作者：
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通讯作者：
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