A transient decrease in spleen size following stroke corresponds to splenocyte release into systemic circulation.
A transient decrease in spleen size following stroke corresponds to splenocyte release into systemic circulation.
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DOI：
10.1007/s11481-012-9406-8
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发表时间：
2012-12
影响因子：
6.2
通讯作者：
Pennypacker, Keith R.
中科院分区：
文献类型：
--
作者：
Seifert, Hilary A.;Hall, Aaron A.;Chapman, Cortney B.;Collier, Lisa A.;Willing, Alison E.;Pennypacker, Keith R.
脾对中风的反应是对缺血性损伤的促炎反应，导致扩大的神经变性。脾切除术减少了啮齿动物出血性和缺血性中风模型中的神经损伤，然而这种反应的确切性质尚未完全了解。本研究利用羧基荧光素二乙酸琥珀酰亚胺酯（CFSE）在体内标记脾细胞，观察脑缺血后脾细胞的迁移。与假手术对照组相比，大鼠大脑中动脉闭塞（MCAO）后24至48 h，脾脏的大小显著减小。MCAO后96小时，脾脏大小恢复到与假手术大鼠无差异的水平。为了追踪MCAO后的脾细胞迁移，用CFSE注射脾以标记细胞。与48 h假手术组相比，48 h MCAO组的CFSE阳性细胞数量显著减少，96 h MCAO组和假手术组的CFSE标记细胞数量相当。标记的淋巴细胞，单核细胞和中性粒细胞的显着增加，检测到在48小时后，MCAO的血液相比，其他组。CFSE标记的细胞在MCAO后迁移到大脑，但似乎保留在血管系统内。在MCAO后48 h和96 h，这些细胞被鉴定为自然杀伤细胞（NK细胞）和单核细胞，NK细胞，T细胞和单核细胞。缺血性损伤后，脾细胞进入体循环并迁移到大脑，加剧神经变性。
The splenic response to stroke is a proinflammatory reaction to ischemic injury resulting in expanded neurodegeneration. Splenectomy reduces neural injury in rodent models of hemorrhagic and ischemic stroke, however the exact nature of this response has yet to be fully understood. This study examines the migration of splenocytes after brain ischemia utilizing carboxyfluorescein diacetate succinimidyl ester (CFSE) to label them in vivo. The spleen was found to significantly decrease in size from 24 to 48 h following middle cerebral artery occlusion (MCAO) in rats compared to sham operated controls. By 96 h post-MCAO the spleen size returned to levels not different from sham operated rats. To track splenocyte migration following MCAO, spleens were injected with CFSE to label cells. CFSE positive cell numbers were significantly reduced in the 48 h MCAO group versus 48 h sham and CFSE labeled cells were equivalent in 96 h MCAO and sham groups. A significant increase of labeled lymphocyte, monocytes, and neutrophils was detected in the blood at 48 h post-MCAO when compared to the other groups. CFSE labeled cells migrated to the brain following MCAO but appear to remain within the vasculature. These cells were identified as natural killer cells (NK) and monocytes at 48 h and at 96 h post-MCAO NK cells, T cells and monocytes. After ischemic injury, splenocytes enter into systemic circulation and migrate to the brain exacerbating neurodegeneration.
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影响因子：
14.5
作者：
Lee, Soon-Tae;Chu, Kon;Roh, Jae-Kyu
通讯作者：
Roh, Jae-Kyu
影响因子：
2.1
作者：
Ajmo, Craig T., Jr.;Vernon, Dionne O. L.;Cuevas, Javier
通讯作者：
Cuevas, Javier
影响因子：
2.9
作者：
Duckworth, EAM;Butler, TL;Pennypacker, KR
通讯作者：
Pennypacker, KR
DOI：
10.1097/00004647-199607000-00007
发表时间：
1996-07-01
影响因子：
6.3
作者：
Chopp, M;Li, Y;Prostak, J
通讯作者：
Prostak, J
影响因子：
4.8
作者：
Kanemoto, Y;Nakase, H;Sakaki, T
通讯作者：
Sakaki, T