High-resolution view of the yeast meiotic program revealed by ribosome profiling.
High-resolution view of the yeast meiotic program revealed by ribosome profiling.
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DOI：
10.1126/science.1215110
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发表时间：
2012-02-03
期刊：
影响因子：
--
通讯作者：
Weissman JS
中科院分区：
文献类型：
--
作者：
Brar GA;Yassour M;Friedman N;Regev A;Ingolia NT;Weissman JS
减数分裂是一个复杂的发育过程，由二倍体祖细胞产生单倍体细胞。我们通过酵母减数分裂产孢程序测量了信使RNA （mRNA）丰度和蛋白质产量，发现大多数基因通过控制mRNA水平和翻译效率实现了强烈的阶段特异性表达。监测蛋白质生产时间揭示了非特征重组因子和广泛的细胞器重塑。减数分裂翻译也向非规范位点转移，包括未注释转录本上的短开放阅读框（orf）和已知转录本的上游区域（uorf）。核糖体占据近同源的ORF位点与ORF翻译效率更高有关；相比之下，一些经常受到监管的5 ' leader扩展影响的AUG uorf表现出了竞争性。这项工作揭示了在减数分裂中普遍存在的翻译控制，并有助于阐明减数分裂细胞广泛重组的分子基础。
Meiosis is a complex developmental process that generates haploid cells from diploid progenitors. We measured messenger RNA (mRNA) abundance and protein production through the yeast meiotic sporulation program and found strong, stage-specific expression for most genes, achieved through control of both mRNA levels and translational efficiency. Monitoring of protein production timing revealed uncharacterized recombination factors and extensive organellar remodeling. Meiotic translation is also shifted toward noncanonical sites, including short open reading frames (ORFs) on unannnotated transcripts and upstream regions of known transcripts (uORFs). Ribosome occupancy at near-cognate uORFs was associated with more efficient ORF translation; in contrast, some AUG uORFs, often exposed by regulated 5′ leader extensions, acted competitively. This work reveals pervasive translational control in meiosis and helps to illuminate the molecular basis of the broad restructuring of meiotic cells.
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