Rapid evolution of bacterial mutualism in the plant rhizosphere.
Rapid evolution of bacterial mutualism in the plant rhizosphere.
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DOI：
10.1038/s41467-021-24005-y
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发表时间：
2021-06-22
影响因子：
16.6
通讯作者：
Jousset A
中科院分区：
文献类型：
--
作者：
Li E;de Jonge R;Liu C;Jiang H;Friman VP;Pieterse CMJ;Bakker PAHM;Jousset A
虽然有益的植物-微生物相互作用在自然界中很常见，但细菌共生进化的直接证据却很少。在这里，我们使用实验进化因果关系表明，最初的植物拮抗假单胞菌protegens细菌在6个植物生长周期（6个月）内在拟南芥根际进化为共生菌。这种进化转变伴随着通过两种机制增加的共生适应性：(i)提高了对根分泌物的竞争力；（ii）增强了对植物分泌的抗菌东莨菪素的耐受性，其产生受转录因子MYB72调节。至关重要的是，这些相互适应与植物毒性降低、MYB72在根中的转录增强以及对植物生长的积极影响相结合。遗传上，共生关系与GacS/GacA双组分调控系统的多种突变有关，该系统仅在植物存在时才具有高适应度效益。总之，我们的研究结果表明，根际细菌可以在农业相关的进化时间尺度上沿着寄生-共生连续体快速进化。有益的植物-微生物相互作用在自然界中很常见，但互惠共生进化的直接证据很少。在这里，Li等人通过实验进化了根际细菌，发现它可以在相对较短的时间内进化成共生菌。
While beneficial plant-microbe interactions are common in nature, direct evidence for the evolution of bacterial mutualism is scarce. Here we use experimental evolution to causally show that initially plant-antagonistic Pseudomonas protegens bacteria evolve into mutualists in the rhizosphere of Arabidopsis thaliana within six plant growth cycles (6 months). This evolutionary transition is accompanied with increased mutualist fitness via two mechanisms: (i) improved competitiveness for root exudates and (ii) enhanced tolerance to the plant-secreted antimicrobial scopoletin whose production is regulated by transcription factor MYB72. Crucially, these mutualistic adaptations are coupled with reduced phytotoxicity, enhanced transcription of MYB72 in roots, and a positive effect on plant growth. Genetically, mutualism is associated with diverse mutations in the GacS/GacA two-component regulator system, which confers high fitness benefits only in the presence of plants. Together, our results show that rhizosphere bacteria can rapidly evolve along the parasitism-mutualism continuum at an agriculturally relevant evolutionary timescale. Beneficial plant-microbe interactions are common in nature, but direct evidence for the evolution of mutualism is scarce. Here, Li et al. experimentally evolve a rhizospheric bacterium and find that it can evolve into a mutualist on a relatively short timescale.
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