Storage differentially impacts alloimmunization to distinct red cell antigens following transfusion in mice.
Storage differentially impacts alloimmunization to distinct red cell antigens following transfusion in mice.
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DOI：
10.1111/trf.17251
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发表时间：
2023-03
期刊：
影响因子：
2.9
通讯作者：
Stowell, Sean R. R.
中科院分区：
文献类型：
--
作者：
Maier, Cheryl L. L.;Jajosky, Ryan P. P.;Patel, Seema R. R.;Verkerke, Hans P. P.;Fuller, Megan D. D.;Allen, Jerry William;Zerra, Patricia E. E.;Fasano, Ross M. M.;Chonat, Satheesh;Josephson, Cassandra D. D.;Gibb, David R. R.;Eisenbarth, Stephanie C. C.;Luckey, C. John;Hudson, Krystalyn E. E.;Hendrickson, Jeanne E. E.;Arthur, Connie E. M.;Stowell, Sean R. R.
血液储存对红细胞（RBC）同种免疫的影响仍然存在争议，一些研究表明RBC诱导的同种抗体产生增强，而另一些研究未能观察到储存对同种抗体形成的任何影响。由于对患者红细胞同种异体免疫的储存评价已经检查了针对广泛同种异体抗原的抗体形成，因此不同的临床结局仍然可能反映了储存对特异性抗原同种异体免疫的可变影响。将表达两种不同模型抗原HEL-OVA-Duffy（HOD）和KEL（单独或一起（HOD × KEL））的RBC储存0、8或14天，然后在输血前检测抗原水平。在输血后24小时内评估输注供体RBC存活率，而在输血后5天和14天评估IgM和IgG抗体产生。储存的HOD或KEL RBC分别保持与新鲜RBC相似的HEL或KEL抗原水平，但随着储存年龄的增加，RBC清除率确实增强。储存增强IgG抗体形成对HOD，而相反的结果发生后，输血储存KEL红细胞。储存对HOD或KEL同种免疫的明显影响似乎并不反映HOD或KEL RBC之间的内在差异，因为输注储存的HOD × KEL RBC导致IgG抗HOD抗体形成增加和IgG抗KEL抗体形成减少。这些数据证明了储存对不同RBC抗原免疫的二分影响，为不一致的临床经验提供了可能的解释，并需要对RBC储存和同种异体免疫之间的关系进行额外的研究。
The impact of blood storage on red blood cell (RBC) alloimmunization remains controversial, with some studies suggesting enhancement of RBC-induced alloantibody production and others failing to observe any impact of storage on alloantibody formation. Since evaluation of storage on RBC alloimmunization in patients has examined antibody formation against a broad range of alloantigens, it remains possible that different clinical outcomes reflect a variable impact of storage on alloimmunization to specific antigens. RBCs expressing two distinct model antigens, HEL-OVA-Duffy (HOD) and KEL, separately or together (HOD × KEL), were stored for 0, 8, or 14 days, followed by detection of antigen levels prior to transfusion. Transfused donor RBC survival was assessed within 24 h of transfusion, while IgM and IgG antibody production were assessed 5 and 14 days after transfusion. Stored HOD or KEL RBCs retained similar HEL or KEL antigen levels, respectively, as fresh RBCs, but did exhibit enhanced RBC clearance with increased storage age. Storage enhanced IgG antibody formation against HOD, while the oppositive outcome occurred following transfusion of stored KEL RBCs. The distinct impact of storage on HOD or KEL alloimmunization did not appear to reflect intrinsic differences between HOD or KEL RBCs, as transfusion of stored HOD × KEL RBCs resulted in increased IgG anti-HOD antibody development and reduced IgG anti-KEL antibody formation. These data demonstrate a dichotomous impact of storage on immunization to distinct RBC antigens, offering a possible explanation for inconsistent clinical experience and the need for additional studies on the relationship between RBC storage and alloimmunization.
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影响因子：
2.9
作者：
Stowell, Sean R.;Smith, Nicole H.;Yazer, Mark H.
通讯作者：
Yazer, Mark H.
影响因子：
8
作者：
Nemkov, Travis;Stefanoni, Davide;D'Alessandro, Angelo
通讯作者：
D'Alessandro, Angelo
桥接通道树突状细胞可诱导对输血的红细胞的免疫力。
DOI：
10.1084/jem.20151720
发表时间：
2016-05-30
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Calabro S;Gallman A;Gowthaman U;Liu D;Chen P;Liu J;Krishnaswamy JK;Nascimento MS;Xu L;Patel SR;Williams A;Tormey CA;Hod EA;Spitalnik SL;Zimring JC;Hendrickson JE;Stowell SR;Eisenbarth SC
通讯作者：
Eisenbarth SC
影响因子：
4.4
作者：
Arthur, Connie M.;Patel, Seema R.;Stowell, Sean R.
通讯作者：
Stowell, Sean R.
影响因子：
2.9
作者：
Zalpuri, Saurabh;Schonewille, Henk;Zwaginga, Jaap Jan
通讯作者：
Zwaginga, Jaap Jan