Contribution of trimethylamine N-oxide on the growth and pressure tolerance of deep-sea bacteria
Contribution of trimethylamine N-oxide on the growth and pressure tolerance of deep-sea bacteria
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N-氧化三甲胺对深海细菌生长和耐压能力的贡献
DOI：
10.1007/s00343-019-7377-9
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发表时间：
2018-11
影响因子：
1.6
通讯作者：
Wu Long-Fei
中科院分区：
文献类型：
--
作者：
Yin Qunjian;Zhang Weijia;Li Xuegong;Zhou Lihong;Qi Xiaoqing;Zhang Chan;Wu Long-Fei
氧化三甲胺（TMAO）广泛分布于海洋环境中，在氮的生态地球化学循环中起着重要作用。多种海洋细菌利用TMAO作为碳源和氮源或作为电子受体进行无氧呼吸。根据压力改变呼吸成分是深海细菌的共同特征。不同属的深海细菌都具有高静水压（HHP）诱导的TMAO还原酶，这些还原酶被认为在深海压电球中组成型表达，并促进对鱼类释放的TMAO的快速反应，而鱼类是细菌生长的潜在营养物。然而，深海细菌是否普遍采用这种策略仍然未知。本研究从不同深度的海水、沉积物和端足类动物中分离到237株细菌，分属于变形菌门、拟杆菌门、厚壁菌门和放线菌门等23个属。对74株菌株进行了耐压性和TMAO利用率的测定。结果表明，就我们的样品而言，细菌栖息的深度与其耐压性之间没有明显的相关性。交替单胞菌属、盐单胞菌属、海水单胞菌属、发光杆菌属和弧菌属的一些深海菌株显示出利用TMAO的能力，但分离的不动杆菌属、芽孢杆菌属、短单胞菌属、鼠尾菌属、新鞘氨醇菌属、莱茵氏菌属、鞘氨醇菌属和寡养单胞菌属的菌株均不具有利用TMAO的能力，表明TMAO的利用具有种特异性。此外，我们注意到，TMAO的利用能力不同菌株的同一物种。TMAO对新加勒比海弧菌深海分离株生长的影响大于浅水分离株。本研究首次揭示了TMAO在深海细菌中的应用，并拓展了我们对海洋细菌生理特性的认识。
Trimethylamine N-oxide (TMAO) is widely dispersed in marine environments and plays an important role in the biogeochemical cycle of nitrogen. Diverse marine bacteria utilize TMAO as carbon and nitrogen sources or as electron acceptor in anaerobic respiration. Alteration of respiratory component according to the pressure is a common trait of deep-sea bacteria. Deep-sea bacteria from different genera harbor high hydrostatic pressure (HHP) inducible TMAO reductases that are assumed to be constitutively expressed in the deep-sea piezosphere and facilitating quick reaction to TMAO released from fish which is a potential nutrient for bacterial growth. However, whether deep-sea bacteria universally employ this strategy remains unknown. In this study, 237 bacterial strains affiliated to 23 genera of Proteobacteria, Bacteroidetes, Firmicutes and Actinobacteria were isolated from seawater, sediment or amphipods collected at different depths. The pressure tolerance and the utilization of TMAO were examined in 74 strains. The results demonstrated no apparent correlation between the depth where the bacteria inhabit and their pressure tolerance, regarding to our samples. Several deep-sea strains from the genera ofAlteromonas, Halomonas, Marinobacter, Photobacterium, andVibrioshowed capacity of TMAO utilization, but none of the isolatedAcinebacter, Bacillus, Brevundimonas, Muricauda, Novosphingobium, Rheinheimera, SphingobiumandStenotrophomonasdid, indicating the utilization of TMAO is a species-specific feature. Furthermore, we noticed that the ability of TMAO utilization varied among strains of the same species. TMAO has greater impact on the growth of deep-sea isolates ofVibrio neocaledonicusthan shallow-water isolates. Taken together, the results describe for the first time the TMAO utilization in deep-sea bacterial strains, and expand our understanding of the physiological characteristic of marine bacteria.
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影响因子：
3.7
作者：
Martini S;Al Ali B;Garel M;Nerini D;Grossi V;Pacton M;Casalot L;Cuny P;Tamburini C
通讯作者：
Tamburini C
影响因子：
3.2
作者：
S. Lucas;James Han;A. Lapidus;Jan-Fang Cheng;Lynne A. Goodwin;S. Pitluck;Lin Peters;N. Mikhailova;H. Teshima;J. Detter;Cliff Han;R. Tapia;M. Land;L. Hauser;N. Kyrpides;Natalia N. Ivanova;I. Pagani;P. Vannier;P. Oger;D. Bartlett;K. Noll;T. Woyke;M. Jebbar
通讯作者：
S. Lucas;James Han;A. Lapidus;Jan-Fang Cheng;Lynne A. Goodwin;S. Pitluck;Lin Peters;N. Mikhailova;H. Teshima;J. Detter;Cliff Han;R. Tapia;M. Land;L. Hauser;N. Kyrpides;Natalia N. Ivanova;I. Pagani;P. Vannier;P. Oger;D. Bartlett;K. Noll;T. Woyke;M. Jebbar
影响因子：
4.2
作者：
Dong X;Wang H;Zou P;Chen J;Liu Z;Wang X;Huang J
通讯作者：
Huang J
影响因子：
4
作者：
C. Simon-Colin;G. Raguénès;Joelle Cozien;J. Guezennec
通讯作者：
C. Simon-Colin;G. Raguénès;Joelle Cozien;J. Guezennec
DOI：
10.1002/1097-010x(20010215)289:3
发表时间：
2001-02
期刊：
The Journal of experimental zoology
影响因子：
--
作者：
Li Wang;Myung Sik Yoo;Hae Mook Kang;W. Im;H. Choi;Jan Bogerd;Hyuk Bang Kwon
通讯作者：
Li Wang;Myung Sik Yoo;Hae Mook Kang;W. Im;H. Choi;Jan Bogerd;Hyuk Bang Kwon