Processing and cryopreservation of human ureter tissues for single-cell and spatial transcriptomics assays.
Processing and cryopreservation of human ureter tissues for single-cell and spatial transcriptomics assays.
复制标题
对单细胞和空间转录组学测定的人体输尿管组织的加工和冷冻保存。
DOI：
10.1016/j.xpro.2022.101854
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发表时间：
2022-12-16
期刊：
影响因子：
--
通讯作者：
Ting, Angela H.
中科院分区：
文献类型：
--
作者：
Fink, Emily E.;Sona, Surbhi;Lee, Byron H.;Ting, Angela H.
使用单细胞RNA测序（scRNA-seq）和空间转录组学表征人类输尿管组织的细胞异质性，提供了细胞类型，信号传导网络和潜在的细胞间串扰发育和再生途径的详细图谱。我们描述了一种优化的方案，用于产生，冷冻保存和解冻从输尿管组织中分离的单细胞悬液后cycloplasty的scRNA-seq。此外，我们描述了一种用于冷冻保存人输尿管组织的优化方案，用于10 x Genomics Visium空间基因表达平台。有关本方案使用和执行的完整详细信息，请参见Fink等人。（2022年）。从人输尿管组织中制备高活力的单细胞悬液冻存单细胞悬液的步骤单细胞悬液O.C.T.的计数和质量控制用于空间转录组学的人输尿管组织的包埋出版商说明：进行任何实验方案都需要遵守当地机构的实验室安全和伦理指南。使用单细胞RNA测序（scRNA-seq）和空间转录组学表征人类输尿管组织的细胞异质性，提供了细胞类型，信号传导网络和潜在的细胞间串扰发育和再生途径的详细图谱。我们描述了一种优化的方案，用于产生，冷冻保存和解冻从输尿管组织中分离的单细胞悬液后cycloplasty的scRNA-seq。此外，我们描述了一种用于冷冻保存人输尿管组织的优化方案，用于10 x Genomics Visium空间基因表达平台。
Characterizing the cellular heterogeneity of human ureter tissues using single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics provides a detailed atlas of cell types, signaling networks, and potential cell-cell cross talk underlying developmental and regenerative pathways. We describe an optimized protocol for generating, cryopreserving, and thawing single-cell suspensions from ureter tissues isolated post-cystectomy for scRNA-seq. In addition, we describe an optimized protocol for cryopreserving human ureter tissues for 10x Genomics Visium spatial gene expression platform. For complete details on the use and execution of this protocol, please refer to Fink et al. (2022). Generation of high-viability single-cell suspensions from human ureter tissues Steps to cryopreserve single-cell suspensions Counting and quality control of single-cell suspensions O.C.T. embedding of human ureter tissue for spatial transcriptomics Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Characterizing the cellular heterogeneity of human ureter tissues using single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics provides a detailed atlas of cell types, signaling networks, and potential cell-cell cross talk underlying developmental and regenerative pathways. We describe an optimized protocol for generating, cryopreserving, and thawing single-cell suspensions from ureter tissues isolated post-cystectomy for scRNA-seq. In addition, we describe an optimized protocol for cryopreserving human ureter tissues for 10x Genomics Visium spatial gene expression platform.
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影响因子：
46.9
作者：
Ding, Jiarui;Adiconis, Xian;Levin, Joshua Z.
通讯作者：
Levin, Joshua Z.
DOI：
10.1073/pnas.75.7.3507
发表时间：
1978-01-01
影响因子：
11.1
作者：
BADER, CR;MACLEISH, PR;SCHWARTZ, EA
通讯作者：
SCHWARTZ, EA
DOI：
10.1073/pnas.69.7.1987
发表时间：
1972-01-01
影响因子：
11.1
作者：
LAM, DMK
通讯作者：
LAM, DMK
影响因子：
13.6
作者：
Wu, Haojia;Kirita, Yuhei;Humphreys, Benjamin D.
通讯作者：
Humphreys, Benjamin D.
影响因子：
11.8
作者：
Fink, Emily E.;Sona, Surbhi;Tran, Uyen;Desprez, Pierre-Emmanuel;Bradley, Matthew;Qiu, Hong;Eltemamy, Mohamed;Wee, Alvin;Wolkov, Madison;Nicolas, Marlo;Min, Booki;Haber, Georges-Pascal;Wessely, Oliver;Lee, Byron H.;Ting, Angela H.
通讯作者：
Ting, Angela H.