The Molecular Immunology of Complex Carbohydrates
The Molecular Immunology of Complex Carbohydrates
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复杂碳水化合物的分子免疫学
DOI：
--
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发表时间：
1988
影响因子：
--
通讯作者：
M. R. Fedde
中科院分区：
文献类型：
--
作者：
Ephraim Katchalski;D. Kritchevsky;Carl R. Honig;T. K. Goldstick;F. Bruley;Raymond Vanholder;S. G. Massry;Z. Atassi;M. R. Fedde
使用凝集素富集各种淋巴细胞亚群进行了研究。羊蹄甲凝集素（BPA）对B细胞的富集是有效的，用扁豆凝集素进一步分离B细胞，使之对硫酸葡聚糖有高、低反应。还检查了各种凝集素选择性诱导抑制性T细胞活性或辅助性T细胞活性的能力。因此，诱导的抑制性T细胞富集花生凝集素，相反，辅助性T细胞富集鲎凝集素。该方法被应用于分析自身免疫易感小鼠中抑制性和辅助性T细胞水平的年龄依赖性变化。用双花扁豆凝集素（DBA）富集异基因混合淋巴细胞培养物中诱导的细胞毒性T细胞。这些细胞毒性T细胞在体外显示出特异性杀伤作用。然而，当荷瘤小鼠的脾细胞通过使用DBA分级分离时，DBA细胞介导了体内肿瘤的消退。BPA也被发现对产生白细胞介素-2的T细胞亚群和巨噬细胞前体细胞的富集有效。使用该技术，发现自身免疫易感MRL/l小鼠的骨髓细胞富含巨噬细胞前体细胞。
The use of lectins for the enrichment of various lymphocyte subpopulations was investigated. Bauhinia purpurea lectin (BPA) was found to be effective for the enrichment of B cells, and the B cells thus obtained were further fractionated with lentil lectin into subsets showing high and low responces to dextran sulfate. The ability of various lectins to selectively induce suppressor T cell activity or helper T cell activity was also examined. The suppressor T cells thus induced were enriched peanut lectin and, conversely, the helper T cells were enriched with Limulus polyhemus lectin. This method was applied to analysis of age-dependent changes in the levels of suppressor and helper T cells in autoimmune-prone mice. Cytotoxic T cells induced in an allogeneic mixed lymphocyte culture were enriched with Dolichos biflorus lectin (DBA). These cytotoxic T cells showed a specific killing effect in vitro. However, when spleen cells of tumor-bearing mice were fractionated by use of DBA, the DBAcells mediated the regression of the tumors in vivo. BPA was also found to be effective for the enrichment of the interleukin-2-producing T cell subset and macrophage precursor cells. Using this technique, bone marrow cells of autoimmune-prone MRL/l mice were found to be rich in macrophage precursor cells.
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DOI：
10.1016/s0021-9258(19)45389-1
发表时间：
1982-12
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
John L. MagnaniS;Bo Nilssong;Manfred BrockhausSV;David Zopfl;Zenon SteplewskiJI;Hilary Koprowskill;V. Ginsburg
通讯作者：
John L. MagnaniS;Bo Nilssong;Manfred BrockhausSV;David Zopfl;Zenon SteplewskiJI;Hilary Koprowskill;V. Ginsburg
DOI：
10.1073/pnas.82.4.1199
发表时间：
1985-02
影响因子：
11.1
作者：
H. Clausen;S. Levery;E. Nudelman;S. Tsuchiya;S. Hakomori
通讯作者：
H. Clausen;S. Levery;E. Nudelman;S. Tsuchiya;S. Hakomori
ABH 和 Lewis 组合抗原的淋巴细胞毒性定义及其从血清到淋巴细胞的转移。
影响因子：
2.7
作者：
Oriol,R;Danilovs,J;Lemieux,R;Terasaki,P;Bernoco,D
通讯作者：
Bernoco,D
人乳腺上皮细胞分泌的蛋白聚糖中存在 O-连接寡糖的结构。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Gowda,DC;Bhavanandan,VP;Davidson,EA
通讯作者：
Davidson,EA
培养中未转化和转化的牛角膜内皮细胞产生的细胞外基质相关糖胺聚糖的表征。
DOI：
10.1111/j.1432-1033.1985.tb09277.x
发表时间：
1985
期刊：
European journal of biochemistry
影响因子：
--
作者：
Wang,ZW;Irimura,T;Nakajima,M;Belloni,PN;Nicolson,GL
通讯作者：
Nicolson,GL