Gene cloning and expression analysis of AhR and CYP4 from Pinctada martensii after exposed to pyrene
Gene cloning and expression analysis of AhR and CYP4 from Pinctada martensii after exposed to pyrene
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马氏珠母贝AhR和CYP4芘暴露后基因克隆及表达分析
DOI：
10.1007/s10646-015-1424-x
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发表时间：
2015-02
期刊：
影响因子：
2.7
通讯作者：
Fuqiang Wang
中科院分区：
文献类型：
--
作者：
Xiaoping Diao;Yuhu Li;Pengfei Zheng;Fuqiang Wang
芘是一种典型的多环芳烃，是海洋环境中常见的污染物。多环芳烃通过激活芳基烃受体（AhR）在暴露的生物体中启动细胞解毒。这些外源性药物的后续代谢主要是通过I期解毒系统的细胞色素P450酶进行的。克隆了马氏珠贝（Pinctada martensii, pm）编码AhR和细胞色素P4的全长互补DNA序列。马氏拟虫AhR互补DNA序列构成一个开放阅读框，编码848个氨基酸。序列分析表明，PmAhR与其他双壳类物种的同源物具有较高的相似性。马氏疟原虫细胞色素P(CYP)4互补DNA序列构成一个开放阅读框，编码489个氨基酸。实时定量分析检测了暴露于芘的马氏p.a tensii的套膜、鳃、肝胰腺和内收肌中PmAhR和PmCYP4信使RNA的表达。PmAhR和PmCYP4的转录带强度在鳃中最高。PmAhR和PmCYP4信使rna诱导的时间表达在鳃中观察到，并在暴露后3至5天增加；然后回到控制水平。这些结果表明，珍珠牡蛎PmAhR和PmCYP4信使rna可能是监测海洋环境芘污染的有用参数。
Pyrene, a typical polycyclic aromatic hydrocarbon, is a common pollutant in the marine environment. Polycyclic aromatic hydrocarbons initiate cellular detoxification in an exposed organism via the activation of the aryl hydrocarbon receptor (AhR). Subsequent metabolism of these xenobiotics is mainly by the cytochrome P450 enzymes of the phase I detoxification system. Full-length complementary DNA sequences from the pearl oyster Pinctada martensii (pm) encoding AhR and cytochrome P4 were cloned. The P. martensii AhR complementary DNA sequence constitutes an open reading frame that encodes for 848 amino acids. Sequence analysis indicated PmAhR showed high similarity with its homologues of other bivalve species. The cytochrome P(CYP)4 complementary DNA sequence of P. martensii constitutes an open reading frame that encodes for 489 amino acids. Quantitative real-time analysis detected both PmAhR and PmCYP4 messenger RNA expressions in the mantle, gill, hepatapancreas and adductor muscle of P. martensii exposed to pyrene. The highest transcript-band intensities of PmAhR and PmCYP4 were observed in the gill. Temporal expression of PmAhR and PmCYP4 messenger RNAs induction was observed in gills and increased between 3 and 5 days post exposure; then returned to control level. These results suggest that messenger RNAs of PmAhR and PmCYP4 in pearl oysters might be useful parameters for monitoring marine environment pyrene pollution.
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影响因子：
3.3
作者：
Shuangmei Tian;L. Pan;Hui Zhang
通讯作者：
Shuangmei Tian;L. Pan;Hui Zhang
影响因子：
1.9
作者：
Jingjing Miao;L. Pan;Na Liu;Chaoqun Xu;Lin Zhang
通讯作者：
Jingjing Miao;L. Pan;Na Liu;Chaoqun Xu;Lin Zhang
DOI：
10.1016/j.cca.2004.05.007
发表时间：
2004-05
期刊：
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
影响因子：
--
作者：
K. Rewitz;C. Kjellerup;A. Jørgensen;C. Petersen;O. Andersen
通讯作者：
K. Rewitz;C. Kjellerup;A. Jørgensen;C. Petersen;O. Andersen
DOI：
10.1002/j.1460-2075.1993.tb06101.x
发表时间：
1993-11
期刊：
The EMBO Journal
影响因子：
--
作者：
M. Whitelaw;M. Göttlicher;J. Gustafsson;L. Poellinger
通讯作者：
M. Whitelaw;M. Göttlicher;J. Gustafsson;L. Poellinger
影响因子：
4.3
作者：
L. Pan;Na Liu;Chaoqun Xu;Jingjing Miao
通讯作者：
L. Pan;Na Liu;Chaoqun Xu;Jingjing Miao