Bioassay research methodology: measuring CRH in pregnancy.
Bioassay research methodology: measuring CRH in pregnancy.
复制标题
DOI：
10.1177/1099800408320970
复制
发表时间：
2008-07
影响因子：
2.5
通讯作者：
Ruiz RJ
中科院分区：
文献类型：
--
作者：
Latendresse G;Ruiz RJ
有文献证明母体促肾上腺皮质激素释放激素（CRH）水平升高与不良妊娠结局之间存在关联。然而，这些发现的报告往往缺乏关于生物测定方法的足够细节和基本原理。对于那些不具备深入的实验室科学知识但又希望将生物测定纳入其调查或评估已发表报告的研究人员来说，这一缺点可能是有问题的。CRH测量结果的质量和可靠性会受到样品收集、处理、储存和生物测定过程中遇到的变量的显著影响。因此，重要的是建立基于充分知情的理由的研究实验室协议，并仔细考虑和控制相关变量。综合实验室科学文献关于变量影响CRH测量在妊娠提出。此外，与经验丰富的研究人员的咨询提供了对CRH测量的深入了解。根据这些资料，制定了临床研究的实验室规程。已经确定了与妊娠期CRH测量可靠性相关的多个变量。这些包括样品收集方法，样品处理，样品完整性，样品储存和实际测定选择。通过识别和控制样品收集、处理、储存和生物测定过程中遇到的变量，可以显著提高CRH测量的可靠性。仅从已发表的文献中很难收集到足够的方法细节，因此与消息灵通的研究人员协商是必要的。提出了CRH生物测定在临床研究中的应用方案。
There are documented associations between elevated maternal corticotropin-releasing hormone (CRH) levels and adverse pregnancy outcomes. However, reports of these findings often lack sufficient detail and rationale regarding the bioassay methodology. This shortcoming can be problematic for researchers who do not possess in-depth laboratory sciences knowledge but who want to include bioassays in their investigations or to evaluate published reports. The quality and reliability of CRH measurement results can be significantly affected by variables encountered during sample collection, processing, storage, and bioassay. Thus, it is important to establish research laboratory protocols that are based on well-informed rationales and to carefully consider and control for relevant variables. A synthesis of laboratory sciences literature regarding variables affecting CRH measurement in pregnancy is presented. Additionally, consultation with experienced researchers provided an in-depth understanding of CRH measurement. From these sources, a laboratory protocol for clinical research was developed. Multiple variables that are specific to the reliability of CRH measurement in pregnancy have been identified. These include sample collection methods, sample processing, sample integrity, sample storage, and the actual assay selected. The reliability of CRH measurements can be significantly improved by identifying and controlling for variables encountered during sample collection, processing, storage, and bioassay. Adequate methodological details are difficult to glean solely from the published literature, thus consultation with well-informed researchers is necessary. A protocol for CRH bioassay in clinical research is proposed.
登录
查看更多内容
影响因子：
9.8
作者：
Sibai, B;Meis, PJ;Gabbe, S
通讯作者：
Gabbe, S
影响因子：
4.2
作者：
Hobel, C;Culhane, J
通讯作者：
Culhane, J
影响因子：
5.8
作者：
Behan, DP;Khongsaly, O;deSouza, EB
通讯作者：
deSouza, EB
影响因子：
2.8
作者：
Strong, EF;Kleinman, KP;Rich-Edwards, JW
通讯作者：
Rich-Edwards, JW
DOI：
10.1016/s0002-9378(99)70712-x
发表时间：
1999-01-01
影响因子：
9.8
作者：
Hobel, CJ;Dunkel-Schetter, C;Arora, CP
通讯作者：
Arora, CP