The responses to surface wettability gradients induced by chitosan nanofilms on microtextured titanium mediated by specific integrin receptors.
The responses to surface wettability gradients induced by chitosan nanofilms on microtextured titanium mediated by specific integrin receptors.
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DOI：
10.1016/j.biomaterials.2012.06.066
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发表时间：
2012-10
期刊：
影响因子：
14
通讯作者：
Schwartz, Zvi
中科院分区：
文献类型：
--
作者：
Park, Jung Hwa;Wasilewski, Christine E.;Almodovar, Noelia;Olivares-Navarrete, Rene;Boyan, Barbara D.;Tannenbaum, Rina;Schwartz, Zvi
种植体表面的微观结构和化学性质是调节细胞反应的重要变量。表面化学和润湿性直接相关。虽然这些表面特性中的每一个都可以影响细胞反应，但很难将它们的具体贡献解耦。为了解决这个问题，本研究的目的是开发一种具有特定化学性质的表面润湿性梯度，而不改变微米级粗糙度，并研究表面润湿性对成骨细胞反应的作用。用氧等离子体处理微织构喷砂/酸蚀（SLA，Sa = 3.1 μm）钛圆盘，以增加表面活性氧密度。在从血浆中取出后0、2、6、10和24 h，用壳聚糖涂覆表面30 min，冲洗并干燥。改性的SLA表面表示为在涂覆之前在空气中的SLA/h。表面表征表明，该工艺产生不同的润湿性（SLA 0 <SLA 2 <SLA 10 <SLA 24），而不改变表面的微米级特征。细胞数量减少的润湿性依赖的方式，除了最水润湿的表面，SLA 24。不同润湿性的碱性磷酸酶活性无差异。增加润湿性产生增加的骨钙素和骨保护素的生产，除了在SLA 24表面。整合素α1、α2、α5、β1和β3的mRNA对表面润湿性敏感。然而，表面润湿性不影响整合素α3的mRNA水平。沉默β1以润湿性依赖的方式增加细胞数量，减少骨钙素和骨保护素。表面润湿性作为主要调节因子促进成骨细胞分化，但整合素表达和沉默β1结果表明，表面润湿性通过差异整合素表达谱而不是微纹理来调节成骨细胞。结果可能表明，具有特定化学性质的微观结构和润湿性对骨整合具有重要的调节作用。每种性质具有不同的作用，其由不同的整合素受体介导。
Microtexture and chemistry of implant surfaces are important variables for modulating cellular responses. Surface chemistry and wettability are connected directly. While each of these surface properties can influence cell response, it is difficult to decouple their specific contributions. To address this problem, the aims of this study were to develop a surface wettability gradient with a specific chemistry without altering micron scale roughness and to investigate the role of surface wettability on osteoblast response. Microtextured sandblasted/acid-etched (SLA, Sa = 3.1 μm) titanium disks were treated with oxygen plasma to increase reactive oxygen density on the surface. At 0, 2, 6, 10, and 24 h after removing them from the plasma, the surfaces were coated with chitosan for 30 min, rinsed and dried. Modified SLA surfaces are denoted as SLA/h in air prior to coating. Surface characterization demonstrated that this process yielded differing wettability (SLA0 < SLA2 < SLA10 < SLA24) without modifying the micron scale features of the surface. Cell number was reduced in a wettability-dependent manner, except for the most water-wettable surface, SLA24. There was no difference in alkaline phosphatase activity with differing wettability. Increased wettability yielded increased osteocalcin and osteoprotegerin production, except on the SLA24 surfaces. mRNA for integrins α1, α2, α5, β1, and β3 was sensitive to surface wettability. However, surface wettability did not affect mRNA levels for integrin α3. Silencing β1 increased cell number with reduced osteocalcin and osteoprotegerin in a wettability-dependent manner. Surface wettability as a primary regulator enhanced osteoblast differentiation, but integrin expression and silencing β1 results indicate that surface wettability regulates osteoblast through differential integrin expression profiles than microtexture does. The results may indicate that both microtexture and wettability with a specific chemistry have important regulatory effects on osseointegration. Each property had different effects, which were mediated by different integrin receptors.
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影响因子：
9.7
作者：
Lavenus, Sandrine;Pilet, Paul;Layrolle, Pierre
通讯作者：
Layrolle, Pierre
DOI：
10.1002/jss.400060211
发表时间：
1977-01-01
期刊：
JOURNAL OF SUPRAMOLECULAR STRUCTURE
影响因子：
--
作者：
MORRIS, ER;REES, DA;WELSH, EJ
通讯作者：
WELSH, EJ
影响因子：
14
作者：
Raines AL;Olivares-Navarrete R;Wieland M;Cochran DL;Schwartz Z;Boyan BD
通讯作者：
Boyan BD
DOI：
10.1073/pnas.0805420105
发表时间：
2008-10-14
影响因子：
11.1
作者：
Olivares-Navarrete, R.;Raz, P.;Schwartz, Z.
通讯作者：
Schwartz, Z.
影响因子：
4.9
作者：
Dulgar-Tulloch, A. J.;Bizios, R.;Siegel, R. W.
通讯作者：
Siegel, R. W.