Methionine sulfoxide reductases protect against oxidative stress in Staphylococcus aureus encountering exogenous oxidants and human neutrophils.
Methionine sulfoxide reductases protect against oxidative stress in Staphylococcus aureus encountering exogenous oxidants and human neutrophils.
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DOI：
10.1159/000355915
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发表时间：
2014
影响因子：
5.3
通讯作者：
Nauseef WM
中科院分区：
文献类型：
--
作者：
Pang YY;Schwartz J;Bloomberg S;Boyd JM;Horswill AR;Nauseef WM
为了成功确定感染，金黄色葡萄球菌必须躲避中性粒细胞(PMN)的清除。我们研究了参与氧化葡萄球菌蛋白修复的蛋氨酸亚砜还原酶(MSR)的表达和调控，并探讨了它们对暴露于氧化剂或PMN的金黄色葡萄球菌命运的影响。我们评估了一个msrA1和msrB缺失的突变体对过氧化氢、次氯酸和PMN的敏感性。实时荧光定量聚合酶链式反应检测人PMN吞噬野生型细菌中msrA1的表达。通过筛选双组分调节系统(TCS)突变体文库，研究MSR的调节。与野生型相比，MSR缺失的细菌对氧化剂和PMN更敏感。葡萄球菌msrA1的上调发生在正常PMN和NADPH氧化酶活性缺乏的PMN的吞噬小体中。此外，PMN富含颗粒提取物还能刺激msrA1表达上调。PMN内msrA1的调制部分依赖于VraSR TCS。MSR有助于葡萄球菌对氧化攻击和PMN的反应。我们的研究突出了氧化蛋白修复途径和VraSR TCS之间的一种新的相互作用，VraSR TCS涉及细胞壁的动态平衡。
To establish infection successfully, S. aureus must evade clearance by polymorphonuclear neutrophils (PMN). We studied the expression and regulation of the methionine sulfoxide reductases (Msr) that are involved in the repair of oxidized staphylococcal proteins and investigated their influence over the fate of S. aureus exposed to oxidants or PMN. We evaluated a mutant deficient in msrA1 and msrB for susceptibility to hydrogen peroxide, hypochlorous acid and PMN. The expression of msrA1 in wild-type bacteria ingested by human PMN was assessed by real-time PCR. The regulation of msr was studied by screening a library of two-component regulatory system (TCS) mutants for altered msr responses. Relative to the wild-type, bacteria deficient in Msr were more susceptible to oxidants and to PMN. Upregulation of staphylococcal msrA1 occurred within the phagosomes of normal PMN and PMN deficient in NADPH oxidase activity. Furthermore, PMN granule-rich extract stimulated the upregulation of msrA1. Modulation of msrA1 within PMN was shown to be partly dependent on the VraSR TCS. Msr contributes to staphylococcal responses to oxidative attack and PMN. Our study highlights a novel interaction between the oxidative protein repair pathway and the VraSR TCS that is involved in cell wall homeostasis.
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影响因子：
56.9
作者：
BAEHNER, RL;NATHAN, DG
通讯作者：
NATHAN, DG
影响因子：
15.9
作者：
ODEBERG, H;OLSSON, I
通讯作者：
OLSSON, I
影响因子：
4.2
作者：
Dengler V;Meier PS;Heusser R;Berger-Bächi B;McCallum N
通讯作者：
McCallum N
影响因子：
3.6
作者：
Lee WL;Gold B;Darby C;Brot N;Jiang X;de Carvalho LP;Wellner D;St John G;Jacobs WR Jr;Nathan C
通讯作者：
Nathan C
影响因子：
4.8
作者：
Belcheva, Antoaneta;Golemi-Kotra, Dasantila
通讯作者：
Golemi-Kotra, Dasantila