Spatial and temporal pattern of cellular myc oncogene expression in developing human placenta: Implications for embryonic cell proliferation
Spatial and temporal pattern of cellular myc oncogene expression in developing human placenta: Implications for embryonic cell proliferation
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发育中的人胎盘中细胞癌基因表达的空间和时间模式：对胚胎细胞增殖的影响
DOI：
10.1016/0092-8674(84)90513-0
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发表时间：
1984
期刊：
影响因子：
64.5
通讯作者：
R. Ohlsson
中科院分区：
文献类型：
--
作者：
S. Pfeifer;A. Goustin;J. Rydnert;T. Wahlström;L. Bjersing;D. Stéhelin;R. Ohlsson
我们已经分析了阶段人胎盘的北方，斑点杂交，原位杂交人类c-myc探针。胎盘RNA表现出c-myc基因的2.4 kb转录本的阶段特异性外观。这种转录物的频率在胎盘发育过程中变化20至30倍，在受孕后4-5周显示出峰值，其中myc转录物占总胎盘mRNA重量的约0.05%。在妊娠前三个月结束前，胎盘c-myc转录明显下降。用~（251）标记的myc探针进行的原位杂交显示，myc转录本在胎盘中的空间分布不均匀，在早期胎盘的细胞滋养层细胞壳中表达特别高。标记胎盘外植体与3 H-胸苷，本地化的myc转录的细胞滋养层细胞，和myc表达的时间模式都支持myc转录丰度和细胞滋养层细胞增殖之间的强相关性。我们认为c-myc基因在该组织中的正常细胞增殖中的作用。
We have analyzed staged human placentas by Northern, dot blot, and in situ hybridization to human c-myc probes. Placental RNA exhibits a stage-specific appearance of a 2.4 kb transcript of the c-myc gene. The frequency of this transcript varies 20 to 30 fold over the course of placental development, showing a peak at 4-5 weeks after conception, where the myc transcripts comprise about 0.05% by weight of the total placental mRNA. A clear decline in placental c-myc transcription is seen before the end of the first trimester of pregnancy. In situ hybridization to ‘251-labeled myc probes demonstrates an unequal spatial distribution of myc transcripts in placenta with particularly high expression in the cytotrophoblastic shell of early placenta. Labeling of placental explants with 3H-thymidine, the localization of myc transcripts to cytotrophoblasts, and the temporal pattern of myc expression all support a strong correlation between myc transcript abundance and cytotrophoblast proliferation. We argue for a role for the c-myc gene in the proliferation of normal cells in this tissue.
DOI：
10.1073/pnas.80.20.6307
发表时间：
1983-10
影响因子：
11.1
作者：
R. Watt;K. Nishikura;J. Sorrentino;A. Ar-rushdi;C. Croce;G. Rovera
通讯作者：
R. Watt;K. Nishikura;J. Sorrentino;A. Ar-rushdi;C. Croce;G. Rovera
DOI：
10.1073/pnas.80.3.820
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
ERIKSON, J;ARRUSHDI, A;CROCE, CM
通讯作者：
CROCE, CM