Protein pI alteration related to strain variation of infectious bronchitis virus, an avian Coronavirus.
Protein pI alteration related to strain variation of infectious bronchitis virus, an avian Coronavirus.
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DOI：
10.1016/0166-0934(91)90012-o
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发表时间：
1991-06
影响因子：
3.1
通讯作者：
Chang PW
中科院分区：
文献类型：
--
作者：
Sadasiv EC;Yeh TT;Chang PW
利用等电点（pI）分离了两株具有不同组织营养和血清学特性的传染性支气管炎病毒（IBV）的病毒蛋白。病毒有四种结构蛋白；在血清学上最重要的蛋白质位于叶尖。等电聚焦分离得到的病毒结构蛋白与SDS-PAGE分离得到的病毒结构蛋白进行了比较鉴定。3条蛋白带在pI上相同，1条蛋白带在不同菌株之间存在差异。当将再生的病毒蛋白进行Western blot并与菌株特异性抗血清反应时，仅在菌株特异性变异带对应的点上观察到抗原-抗体络合。对于IBV菌株Mass-41，抗原-抗体复合物的pI值为6.8，而对于IBV菌株Ark-99，抗原-抗体复合物的pI值为7.2。两株抗血清均未见交叉反应。由于组织亲和性是病毒聚合物介导的病毒附着于细胞的功能，并且通常与致病性直接相关，因此似乎可以通过蛋白质pI的改变来检测IBV菌株的致病性改变。根据pI对至少较小病毒的蛋白质进行分类，可以根据变异蛋白质对这些病毒的不可分型、高致病性或持久性毒株进行表征。
Viral proteins of two strains of infectious bronchitis virus (IBV), which have different tissue trophism and serology, were separated on the basis of their isoelectric points (pI). The viruses have four structural proteins; the protein of greatest serological importance is found at the peplomer tip. The viral structural proteins separated by isoelectric focusing were identified by comparison to SDS-PAGE separations. Three protein bands were identical in pI and one protein band showed a difference in pI between strains. When the renatured viral proteins were Western blotted and reacted with strain-specific antiserum, antigen-antibody complexing was seen only at points corresponding to the strain-specific variant bands. For IBV strain Mass-41, antigen-antibody complexing occurred at a pI of 6.8, and, for IBV strain Ark-99, at 7.2. No cross reaction of antisera was observed for either strain. Since tissue affinities are a function of the viral peplomer-mediated attachment of virus to cells and are often directly related to pathogenicity, it appears that altered pathogenicity of strains of IBV may be detected by alteration of pI of the proteins. Classification by pI of proteins of at least the smaller viruses allows untypeable, highly pathogenic or persistent strains of these viruses to be characterized on the basis of variant proteins.
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影响因子：
3.7
作者：
Kusters JG;Niesters HG;Lenstra JA;Horzinek MC;van der Zeijst BA
通讯作者：
van der Zeijst BA
影响因子：
1.4
作者：
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通讯作者：
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1.4
作者：
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3.8
作者：
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通讯作者：
DAVIS, PJ