Metabolomics Study of Serum from a Chronic Alcohol-Fed Rat Model Following Administration of Defatted Tenebrio molitor Larva Fermentation Extract.
Metabolomics Study of Serum from a Chronic Alcohol-Fed Rat Model Following Administration of Defatted Tenebrio molitor Larva Fermentation Extract.
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DOI：
10.3390/metabo10110436
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发表时间：
2020-10-29
期刊：
影响因子：
4.1
通讯作者：
Paik MJ
中科院分区：
文献类型：
--
作者：
Choi RY;Ji M;Lee MK;Paik MJ
我们以前已经表明，脱脂黄粉虫发酵提取物（MWF）减轻酒精性肝损伤，通过调节脂质，炎症和抗氧化剂代谢慢性酒精喂养大鼠。目前的代谢组学研究是通过气相色谱-串联质谱法（GC-MS/MS）对大鼠酒精性肝损伤模型给予MWF（每日一次，持续8周）后监测生化事件。测定血清中15种氨基酸（AAs）、17种有机酸（OAs）和19种游离脂肪酸（FFA）的水平。采用方差分析（ANOVA）、主成分分析（PCA）和偏最小二乘判别分析（PLS-DA）比较血清中51种代谢物的水平。特别地，3-羟基丁酸（3-HB）、焦谷氨酸（PG）、十八烷酸和二十二碳六烯酸（DHA）被评价为如通过PLS-DA和PCA确定的高变量重要性点（VIP）评分和PCA负荷评分，并且这些在MWF和水飞蓟素组中显著高于EtOH组。MWF通过提高肝脏β-氧化活性对酒精性肝损伤具有保护作用，MWF组血清3-HB水平显著高于EtOH对照组。甘氨酸水平在MWF组高于EtOH组，PG水平（与谷胱甘肽产生有关）也升高，表明酒精相关的氧化应激减少。此外，与乙醇组相比，MWF通过增加血清DHA、棕榈酸和十八烷酸水平而免受酒精诱导的炎症和脂肪变性的影响。这些结果表明，MWF可能减轻酒精性肝病，由于其抗炎和抗氧化作用，通过上调肝脏β-氧化活性和下调肝脏FFA摄取。
We have previously showed that defatted mealworm fermentation extract (MWF) attenuates alcoholic liver injury by regulating lipid, inflammatory, and antioxidant metabolism in chronic alcohol-fed rats. The current metabolomics study was performed to monitor biochemical events following the administration of MWF (daily for eight weeks) to a rat model of alcoholic liver injury by gas chromatography-tandem mass spectrometry (GC-MS/MS). The levels of 15 amino acids (AAs), 17 organic acids (OAs), and 19 free fatty acids (FFAs) were measured in serum. Analysis of variance (ANOVA), principal component analysis (PCA), and partial least squares discriminant analysis (PLS-DA) were used to compare the levels of 51 metabolites in serum. In particular, 3-hydroxybutyric acid (3-HB), pyroglutamic acid (PG), octadecanoic acid, and docosahexaenoic acid (DHA) were evaluated as high variable importance point (VIP) scores and PCA loading scores as determined by PLS-DA and PCA, and these were significantly higher in the MWF and silymarin groups than in the EtOH group. MWF showed a protective effect from alcohol-induced liver damage by elevating hepatic β-oxidation activity, and serum 3-HB levels were significantly higher in the MWF group than in the EtOH control group. Glycine levels were higher in the MWF group than in the EtOH group, and PG levels (related to glutathione production) were also elevated, indicating a reduction in alcohol-related oxidative stress. In addition, MWF is protected from alcohol-induced inflammation and steatosis by increasing serum DHA, palmitic, and octadecanoic acid levels as compared with the EtOH group. These results suggest that MWF might attenuate alcoholic liver disease, due to its anti-inflammatory and antioxidant effects by up-regulating hepatic β-oxidation activity and down-regulating liver FFA uptake.
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