A transgenic mouse that reveals cell shape and arrangement during ureteric bud branching.
A transgenic mouse that reveals cell shape and arrangement during ureteric bud branching.
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DOI：
10.1002/dvg.20452
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发表时间：
2009-02
期刊：
影响因子：
1.5
通讯作者：
Costantini, Frank
中科院分区：
文献类型：
--
作者：
Chi, Xuan;Hadjantonakis, Anna-Katerina;Wu, Zaiqi;Hyink, Deborah;Costantini, Frank
了解上皮细胞形态发生的细胞事件是发育生物学中的一个关键问题。在这里，我们描述了一种新的转基因小鼠系，使之有可能可视化个别细胞，特别是在沃尔夫管和输尿管芽，上皮结构，引起收集系统的肾脏。myr-Venus是荧光蛋白Venus的膜相关形式，在Hoxb 7启动子的控制下在输尿管芽谱系中表达。在Hoxb 7/myr-Venus小鼠中，在泌尿生殖系统发育的所有阶段，所有Wolffian管和输尿管芽上皮细胞的轮廓都被强烈标记，从而可以通过新鲜切除或培养的肾脏的共聚焦显微镜容易地观察到单个细胞的形状和排列。该菌株应是非常有用的细胞行为的研究在输尿管芽分支形态发生在野生型和突变小鼠品系。
Understanding the cellular events that underlie epithelial morphogenesis is a key problem in developmental biology. Here we describe a new transgenic mouse line that makes it possible to visualize individual cells specifically in the Wolffian duct and ureteric bud, the epithelial structures that give rise to the collecting system of the kidney. myr-Venus, a membrane–associated form of the fluorescent protein Venus, was expressed in the ureteric bud lineage under the control of the Hoxb7 promoter. In Hoxb7/myr-Venus mice, the outlines of all Wolffian duct and ureteric bud epithelial cells are strongly labeled at all stages of urogenital development, allowing the shapes and arrangements of individual cells to be readily observed by confocal microscopy of freshly excised or cultured kidneys. This strain should be extremely useful for studies of cell behavior during ureteric bud branching morphogenesis in wild type and mutant mouse lines.
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