Histological characteristics of hair follicles at different hair cycle and in vitro modeling of hair follicle-associated cells of yak (Bos grunniens).
Histological characteristics of hair follicles at different hair cycle and in vitro modeling of hair follicle-associated cells of yak (Bos grunniens).
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DOI：
10.3389/fvets.2023.1277586
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发表时间：
2023
影响因子：
3.2
通讯作者：
Wang, Xiaoyan
中科院分区：
文献类型：
--
作者：
Liao, Bo;Cui, Yan;Yu, Sijiu;He, Junfeng;Yang, Xue;Zou, Shengnan;Li, Sijie;Zhao, Pengfei;Xu, Hongwei;Long, Min;Wang, Xiaoyan
关键词：
为了适应高原环境的极端条件，牦牛进化出了浓密的毛发，使它们成为研究毛发生长机制的理想模型。通过对牦牛的研究，我们可以对毛囊如何发育以及它们在具有挑战性的环境中的周期性生长获得有价值的见解。然而，缺乏必要的数据牦牛毛囊组织学和毛囊的体外细胞模型的缺乏作为一个限制，这样的研究目标。本研究以牦牛为动物模型，研究了毛囊周期变化对皮肤组织结构的影响。此外，我们成功地建立了牦牛皮肤毛囊相关细胞的体外模型，包括毛乳头细胞（DPC），前脂肪细胞和成纤维细胞。我们优化了DPC培养的显微切割技术，简化了程序，减少了所需的时间。此外，我们还改进了牦牛前脂肪细胞向成熟脂肪细胞分化的方法，提高了分化效率。牦牛作为一种自然模型的引入为探索毛发生长机制提供了宝贵的研究资源，有助于更深入地了解毛囊生物学和发展再生医学策略。
To adapt to the extreme conditions of plateau environments, yaks have evolved thick hair, making them an ideal model for investigating the mechanisms involved in hair growth. We can gain valuable insights into how hair follicles develop and their cyclic growth in challenging environments by studying yaks. However, the lack of essential data on yak hair follicle histology and the absence of in vitro cell models for hair follicles serve as a limitation to such research objectives. In this study, we investigated the structure of skin tissue during different hair follicle cycles using the yak model. Additionally, we successfully established in vitro models of hair follicle-associated cells derived from yak skin, including dermal papilla cells (DPCs), preadipocytes, and fibroblasts. We optimized the microdissection technique for DPCs culture by simplifying the procedure and reducing the time required. Furthermore, we improved the methodology used to differentiate yak preadipocytes into mature adipocytes, thus increasing the differentiation efficiency. The introduction of yak as a natural model provides valuable research resources for exploring the mechanisms of hair growth and contributes to a deeper understanding of hair follicle biology and the development of regenerative medicine strategies.
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影响因子：
--
作者：
Geyfman M;Plikus MV;Treffeisen E;Andersen B;Paus R
通讯作者：
Paus R
影响因子：
5.5
作者：
Xiong L;Pei J;Wu X;Kalwar Q;Yan P;Guo X
通讯作者：
Guo X
影响因子：
1.2
作者：
Khan, Muznah;Gasser, Stephan
通讯作者：
Gasser, Stephan
影响因子：
4.4
作者：
Bao, Pengjia;Luo, Jiayu;Yan, Ping
通讯作者：
Yan, Ping
DOI：
10.1073/pnas.1309970110
发表时间：
2013-12-03
影响因子：
11.1
作者：
Higgins, Claire A.;Chen, James C.;Christiano, Angela M.
通讯作者：
Christiano, Angela M.