Comparison of knee injury threshold during tibial compression based on limb orientation in mice.
Comparison of knee injury threshold during tibial compression based on limb orientation in mice.
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DOI：
10.1016/j.jbiomech.2018.04.014
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发表时间：
2018-06-06
影响因子：
2.4
通讯作者：
Christiansen BA
中科院分区：
文献类型：
--
作者：
Hsia AW;Tarke FD;Shelton TJ;Tjandra PM;Christiansen BA
我们之前的研究使用胫骨压缩过载来诱导小鼠前十字韧带（ACL）断裂，而其他研究则应用了类似或更大的压缩强度而没有造成损伤。造成伤害阈值差异的原因尚不清楚。在这项研究中，我们使用“俯卧配置”和“仰卧配置”来比较膝关节损伤阈值，这两种配置在髋关节、膝关节和踝关节屈曲方面有所不同，并使用不同的固定装置来稳定膝关节。雌性和雄性 C57BL/6 小鼠的右肢使用俯卧配置加载，而左肢使用仰卧配置加载。小鼠接受 2-20 N 的渐进加载，或 9 N 或 14 N 的循环加载（n=9-11/性别/加载方法）。俯卧配置的渐进加载导致 ACL 断裂，女性平均为 10.2±0.9 N，男性平均为 11.4±0.7 N。相比之下，仰卧位渐进加载仅导致 36% 的雌性小鼠和 50% 的雄性小鼠发生 ACL 断裂。俯卧配置的循环加载导致 ACL 断裂，在 9 N 时，女性为 15±8 个周期，男性为 24±27 个周期，并且男女均在 14 N 的第一个周期内始终发生。相比之下，仰卧配置的循环加载能够在 9 N 下完成 1,200 个周期，且男女均不会受伤，并且在 14 时，女性平均为 45±41 个周期，男性为 49±25 个周期。 ACL 断裂前 N。这些结果表明，胫骨压缩配置可以强烈影响加载期间的膝关节损伤阈值。
Our previous studies used tibial compression overload to induce anterior cruciate ligament (ACL) rupture in mice, while others have applied similar or greater compressive magnitudes without injury. The causes of these differences in injury threshold are not known. In this study, we compared knee injury thresholds using a “prone configuration” and a “supine configuration” that differed with respect to hip, knee, and ankle flexion, and utilized different fixtures to stabilize the knee. Right limbs of female and male C57BL/6 mice were loaded using the prone configuration, while left limbs were loaded using the supine configuration. Mice underwent progressive loading from 2–20 N, or cyclic loading at 9 N or 14 N (n=9–11/sex/loading method). Progressive loading with the prone configuration resulted in ACL rupture at an average of 10.2±0.9 N for females and 11.4±0.7 N for males. In contrast, progressive loading with the supine configuration resulted in ACL rupture in only 36% of female mice and 50% of male mice. Cyclic loading with the prone configuration resulted in ACL rupture after 15±8 cycles for females and 24±27 cycles for males at 9 N, and always during the first cycle for both sexes at 14 N. In contrast, cyclic loading with the supine configuration was able to complete 1,200 cycles at 9 N without injury for both sexes, and an average of 45±41 cycles for females and 49±25 cycles for males at 14 N before ACL rupture. These results show that tibial compression configurations can strongly affect knee injury thresholds during loading.
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影响因子：
4.6
作者：
Rai MF;Duan X;Quirk JD;Holguin N;Schmidt EJ;Chinzei N;Silva MJ;Sandell LJ
通讯作者：
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