Klf10 regulates odontoblast differentiation and mineralization via promoting expression of dentin matrix protein 1 and dentin sialophosphoprotein genes.
Klf10 regulates odontoblast differentiation and mineralization via promoting expression of dentin matrix protein 1 and dentin sialophosphoprotein genes.
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Klf10 通过促进牙本质基质蛋白 1 和牙本质唾液酸磷蛋白基因的表达来调节成牙本质细胞分化和矿化。
DOI：
10.1007/s00441-015-2260-2
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发表时间：
2016-02
影响因子：
3.6
通讯作者：
Chen, Shuo
中科院分区：
文献类型：
--
作者：
Chen, Zhuo;Li, Wentong;Wang, Han;Wan, Chunyan;Luo, Daoshu;Deng, Shuli;Chen, Hui;Chen, Shuo
Klf10是Krüppel样转录因子家族的成员，在成骨细胞分化、骨形成和矿化过程中起关键作用。然而，Klf10是否参与成牙本质细胞分化和牙齿发育尚未确定。在本研究中，我们研究了Klf10在体内小鼠牙齿发育过程中的表达模式及其在体外成牙本质细胞分化中的作用。Klf10蛋白在小鼠磨牙形成早期和晚期的成釉细胞、成釉细胞和成牙本质细胞中均有表达。此外，Klf10、Dmp1、Dspp和Runx2在小鼠牙乳头间充质分化和矿化过程中的表达显著升高。碱性磷酸酶染色和茜素红S法检测KLF10过表达诱导牙乳头间充质细胞分化和矿化。KLF10还上调小鼠牙乳头间充质细胞成牙本质分化标志基因Dmp1、Dspp和Runx2的表达。因此，KLF10控制Dmp1和Dspp表达的分子机制是以剂量依赖的方式激活它们的调节区。我们的结果提示Klf10参与牙齿发育，并通过上调Dmp1和Dspp转录促进成牙本质细胞分化。
Klf10, a member of the Krüppel-like family of transcription factors, is critical for osteoblast differentiation, bone formation and mineralization. However, whether Klf10 is involved in odontoblastic differentiation and tooth development has not been determined. In this study, we investigate the expression patterns of Klf10 during murine tooth development in vivo and its role in odontoblastic differentiation in vitro. Klf10 protein was expressed in the enamel organ and the underlying mesenchyme, ameloblasts and odontoblasts at early and later stages of murine molar formation. Furthermore, the expression of Klf10, Dmp1, Dspp and Runx2 was significantly elevated during the process of mouse dental papilla mesenchymal differentiation and mineralization. The overexpression of Klf10 induced dental papilla mesenchymal cell differentiation and mineralization as detected by alkaline phosphatase staining and alizarin red S assay. Klf10 additionally up-regulated the expression of odontoblastic differentiation marker genes Dmp1, Dspp and Runx2 in mouse dental papilla mesenchymal cells. The molecular mechanism of Klf10 in controlling Dmp1 and Dspp expression is thus to activate their regulatory regions in a dosage-dependent manner. Our results suggest that Klf10 is involved in tooth development and promotes odontoblastic differentiation via the up-regulation of Dmp1 and Dspp transcription.
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影响因子：
4.8
作者：
Cook, T;Gebelein, B;Urrutia, R
通讯作者：
Urrutia, R
影响因子：
4.1
作者：
Bensamoun, Sabine F.;Hawse, John R.;Spelsberg, Thomas C.
通讯作者：
Spelsberg, Thomas C.
影响因子：
4
作者：
Johnsen, SA;Subramaniam, M;Spelsberg, TC
通讯作者：
Spelsberg, TC
影响因子：
3.5
作者：
Chalaux, E;López-Rovira, T;Ventura, F
通讯作者：
Ventura, F
影响因子：
12.3
作者：
Kaczynski J;Cook T;Urrutia R
通讯作者：
Urrutia R