Phosphatidylethanol formation in human platelets: evidence for thrombin-induced activation of phospholipase D.

Phosphatidylethanol formation in human platelets: evidence for thrombin-induced activation of phospholipase D.
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人血小板中磷脂酰乙醇的形成:凝血酶诱导磷脂酶 D 激活的证据。

DOI:
10.1016/s0006-291x(88)80744-7
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发表时间:
1988
影响因子:
3.1
通讯作者:
Rubin,R
Rubin,R
中科院分区:
生物学4区
文献类型:
--
作者:
Rubin,R

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在(~3H)花生四烯酸标记的人血小板上观察了磷脂酰乙醇的形成。在乙醇存在下,凝血酶以时间和浓度依赖的方式诱导(~3H)磷脂酰乙醇的形成。磷脂酰乙醇的出现不涉及新的合成,因为在32P标记的血小板中或在有32P-ATP存在的情况下被皂苷渗透的血小板中,NO32P放射性被结合到磷脂酰乙醇中。这些数据为凝血酶直接激活人血小板中磷脂酶D提供了证据。
Phosphatidylethanol formation was examined in (3H)arachidonic acid-labeled human platelets. In the presence of ethanol, thrombin induced the formation of (3H)phosphatidylethanol in a time- and concentration-dependent manner. The appearance of phosphatidylethanol did not involvede novosynthesis since no32P radioactivity was incorporated into phosphatidylethanol in32P-labeled platelets or in platelets that were permeabilized with saponin in the presence of32P-ATP. The data provide evidence for the direct activation by thrombin of phospholipase D in human platelets.
DOI: 10.1016/0006-291x(88)90528-1
发表时间: 1988
影响因子: 3.1
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DOI: --
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