Garlic (Allium sativum L.) fertility: transcriptome and proteome analyses provide insight into flower and pollen development.
Garlic (Allium sativum L.) fertility: transcriptome and proteome analyses provide insight into flower and pollen development.
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DOI：
10.3389/fpls.2015.00271
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发表时间：
2015
影响因子：
5.6
通讯作者：
Kamenetsky R
中科院分区：
文献类型：
--
作者：
Shemesh-Mayer E;Ben-Michael T;Rotem N;Rabinowitch HD;Doron-Faigenboim A;Kosmala A;Perlikowski D;Sherman A;Kamenetsky R
作为一种受欢迎的调味品，大蒜的商业品种是不育的，这使得这种植物的遗传研究和育种具有挑战性。然而，最近的育性恢复使这种重要作物的先进生理和遗传研究和杂交成为可能。形态生理学研究，结合转录组和蛋白质组分析以及定量PCR验证，能够鉴定可育和雄性不育大蒜基因型配子体发生的基因和特定过程。两种基因型在花药发育的早期阶段都表现出正常的减数分裂，但在雄性不育植物中，小孢子释放后绒毡层肥大导致花粉退化。转录组分析和全球基因表达谱显示，在可育花和雄性不育花中，有16000个基因存在差异表达。蛋白质组学分析和2d凝胶蛋白图谱的定量比较显示36个显著不同的蛋白点，其中9个仅存在于雄性不育基因型中。生物信息学和定量PCR验证了10个候选基因在雄性不育花和可育花中的表达差异显著。可育和不育大蒜花的形态生理和分子特征的比较表明，呼吸限制和/或绒毡层不受调节的程序性细胞死亡可导致能量不足和随后的花粉败育。提出了大蒜雄性育性和不育的潜在分子标记。
Commercial cultivars of garlic, a popular condiment, are sterile, making genetic studies and breeding of this plant challenging. However, recent fertility restoration has enabled advanced physiological and genetic research and hybridization in this important crop. Morphophysiological studies, combined with transcriptome and proteome analyses and quantitative PCR validation, enabled the identification of genes and specific processes involved in gametogenesis in fertile and male-sterile garlic genotypes. Both genotypes exhibit normal meiosis at early stages of anther development, but in the male-sterile plants, tapetal hypertrophy after microspore release leads to pollen degeneration. Transcriptome analysis and global gene-expression profiling showed that >16,000 genes are differentially expressed in the fertile vs. male-sterile developing flowers. Proteome analysis and quantitative comparison of 2D-gel protein maps revealed 36 significantly different protein spots, 9 of which were present only in the male-sterile genotype. Bioinformatic and quantitative PCR validation of 10 candidate genes exhibited significant expression differences between male-sterile and fertile flowers. A comparison of morphophysiological and molecular traits of fertile and male-sterile garlic flowers suggests that respiratory restrictions and/or non-regulated programmed cell death of the tapetum can lead to energy deficiency and consequent pollen abortion. Potential molecular markers for male fertility and sterility in garlic are proposed.
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影响因子：
--
作者：
CHAUDHURY, AM;LAVITHIS, M;DENNIS, ES
通讯作者：
DENNIS, ES
影响因子：
6.9
作者：
Borg, Michael;Brownfield, Lynette;Twell, David
通讯作者：
Twell, David
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5.8
作者：
Conesa, A;Götz, S;Robles, M
通讯作者：
Robles, M
影响因子：
14.8
作者：
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影响因子：
7.2
作者：
Aarts, MGM;Hodge, R;Pereira, A
通讯作者：
Pereira, A