A prospective study of Romanian agriculture workers for zoonotic influenza infections.
A prospective study of Romanian agriculture workers for zoonotic influenza infections.
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DOI：
10.1371/journal.pone.0098248
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Gray GC
中科院分区：
文献类型：
--
作者：
Coman A;Maftei DN;Krueger WS;Heil GL;Chereches RM;Sirlincan E;Bria P;Dragnea C;Kasler I;Valentine MA;Gray GC
在这项前瞻性研究中，我们试图检查罗马尼亚农业工人中急性人畜共患流感病毒感染的血清流行病学证据。在入组时（2009年）和12个月和24个月时，从312名成年农业工人和51名年龄组匹配的对照中抽取血清。参与者每月联系一次，持续24个月，并询问有关急性流感样疾病（ILI）发作的情况。符合ILI标准的队列成员允许采集呼吸道拭子以及急性和恢复期血清。对9种禽流感病毒、3种猪流感病毒和3种人流感病毒进行了血清学测定。在2年随访期间，共报告了23例ILI事件。两名受试者的标本经rRT-PCR鉴定为甲型流感病毒。在随访期间，3名个体经历了针对3种（各1种）禽流感病毒的微量中和抗体滴度≥1∶80的升高：A/Teal/Hong Kong/w312/97（H6 N1）、A/Hong Kong/1073/1999（H9 N2）或A/Duck/阿尔伯塔/60/1976（H12 N5）。然而，这些参与者都不符合家禽暴露标准。许多受试者证明，三种猪流感病毒（SIV）中至少一种的血凝抑制（HI）试验滴度随时间增加4倍;然而，这三种应答中的两种可能是由于抗人流感的交叉反应抗体所致。只有针对A/Swine/弗兰德斯/1/1998（H3 N2）的抗体滴度升高缺乏这种混淆的证据。在使用多元逻辑回归检查针对该SIV的抗体升高的风险因素时，猪暴露（调整后OR = 1.8，95% CI 1.1-2.8）和烟草使用（调整后OR = 1.8; 95% CI 1.1-2.9）是重要的预测因素。 虽然罗马尼亚最近经历了家禽中高致病性禽流感的多次入侵，但这批罗马尼亚农业工人很少有禽流感病毒感染的证据。相反，有证据表明，特别是在猪暴露的参与者中，感染了人类和一种猪H3 N2流感病毒。
In this prospective study we sought to examine seroepidemiological evidence for acute zoonotic influenza virus infection among Romanian agricultural workers. Sera were drawn upon enrollment (2009) and again at 12 and 24 months from 312 adult agriculture workers and 51 age-group matched controls. Participants were contacted monthly for 24 months and queried regarding episodes of acute influenza-like illnesses (ILI). Cohort members meeting ILI criteria permitted respiratory swab collections as well as acute and convalescent serum collection. Serologic assays were performed against 9 avian, 3 swine, and 3 human influenza viruses. During the two-year follow-up, a total of 23 ILI events were reported. Two subjects' specimens were identified as influenza A by rRT-PCR. During the follow-up period, three individuals experienced elevated microneutralization antibody titers ≥1∶80 against three (one each) avian influenza viruses: A/Teal/Hong Kong/w312/97(H6N1), A/Hong Kong/1073/1999(H9N2), or A/Duck/Alberta/60/1976(H12N5). However, none of these participants met the criteria for poultry exposure. A number of subjects demonstrated four-fold increases over time in hemagglutination inhibition (HI) assay titers for at least one of the three swine influenza viruses (SIVs); however, it seems likely that two of these three responses were due to cross-reacting antibody against human influenza. Only elevated antibody titers against A/Swine/Flanders/1/1998(H3N2) lacked evidence for such confounding. In examining risk factors for elevated antibody against this SIV with multiple logistic regression, swine exposure (adjusted OR = 1.8, 95% CI 1.1–2.8) and tobacco use (adjusted OR = 1.8; 95% CI 1.1–2.9) were important predictors. While Romania has recently experienced multiple incursions of highly pathogenic avian influenza among domestic poultry, this cohort of Romanian agriculture workers had sparse evidence of avian influenza virus infections. In contrast, there was evidence, especially among the swine exposed participants, of infections with human and one swine H3N2 influenza virus.
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影响因子：
11.8
作者：
Jernigan, D. B.;Lindstrom, S. L.;Shaw, M. W.
通讯作者：
Shaw, M. W.
影响因子：
8.8
作者：
Kayali, Ghazi;Setterquist, Sharon F.;Gray, Gregory C.
通讯作者：
Gray, Gregory C.
影响因子：
11.8
作者：
Gray GC;McCarthy T;Capuano AW;Setterquist SF;Olsen CW;Alavanja MC
通讯作者：
Alavanja MC
DOI：
10.3181/00379727-97-23776
发表时间：
1958-01-01
影响因子：
--
作者：
ROWE, WP;HARTLEY, JW;HUEBNER, RJ
通讯作者：
HUEBNER, RJ
影响因子：
11.8
作者：
Gill, James S.;Webby, Richard;Gray, Gregory C.
通讯作者：
Gray, Gregory C.