DISARM is a widespread bacterial defence system with broad anti-phage activities.
DISARM is a widespread bacterial defence system with broad anti-phage activities.
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DOI：
10.1038/s41564-017-0051-0
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发表时间：
2018-01
影响因子：
28.3
通讯作者：
Sorek R
中科院分区：
文献类型：
--
作者：
Ofir G;Melamed S;Sberro H;Mukamel Z;Silverman S;Yaakov G;Doron S;Sorek R
噬菌体捕食对细菌和古细菌施加的进化压力导致了有效的抗噬菌体防御机制的发展，包括限制性修饰和CRISPR-Cas系统。在这里，我们报告了一种新的防御系统，裁军（防御岛系统相关的限制修改），广泛存在于细菌和古细菌中。该基因由5个基因组成，包括一个DNA甲基化酶和另外4个被注释为解旋酶结构域、一个磷脂酶- d （PLD）结构域、一个DUF1998结构域和一个功能未知的基因。将副衣状芽孢杆菌9945A解除武装系统植入枯草芽孢杆菌中，使该工程细菌能够抵抗尾双链DNA噬菌体3个主要家族的噬菌体。通过一系列基因缺失，我们发现5个基因中的4个对disarm介导的防御至关重要，而第5个基因（PLD）对于防御某些噬菌体是多余的。我们进一步表明，解除武装限制进入的噬菌体DNA，并且副苔藓芽孢杆菌解除武装甲基化酶修饰宿主CCWGG基序，作为类似于限制修饰系统的自我DNA标记。我们的研究结果表明，“解除武装”是一种新型的多基因限制修饰模块，扩大了原核生物对抗病毒的防御系统。
The evolutionary pressure imposed by phage predation on bacteria and archaea has resulted in the development of effective anti-phage defence mechanisms, including restriction-modification and CRISPR-Cas systems. Here we report on a new defence system, DISARM (Defence Island System Associated with Restriction-Modification), that is widespread in bacteria and archaea. DISARM is comprised of five genes, including a DNA methylase and four other genes annotated as a helicase domain, a phospholipase-D (PLD) domain, a DUF1998 domain and a gene of unknown function. Engineering the Bacillus paralicheniformis 9945A DISARM system into Bacillus subtilis has rendered the engineered bacteria protected against phages from all 3 major families of tailed double-stranded DNA phages. Using a series of gene deletions we show that four of the five genes are essential for DISARM-mediated defence, with the fifth (PLD) being redundant for defence against some of the phages. We further show that DISARM restricts incoming phage DNA, and that the B. paralicheniformis DISARM methylase modifies host CCWGG motifs as a marker of self DNA akin to restriction-modification systems. Our results suggest that DISARM is a new type of multi-gene restriction-modification module, expanding the arsenal of defence systems known to be at the disposal of prokaryotes against their viruses.
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影响因子：
3.5
作者：
NOYERWEIDNER, M;JENTSCH, S;TRAUTNER, TA
通讯作者：
TRAUTNER, TA
广泛的噬菌体流产感染系统通过IV型毒素 - 抗毒素机制发挥作用。
影响因子：
14.9
作者：
Dy RL;Przybilski R;Semeijn K;Salmond GP;Fineran PC
通讯作者：
Fineran PC
影响因子：
14.8
作者：
Kelley LA;Mezulis S;Yates CM;Wass MN;Sternberg MJ
通讯作者：
Sternberg MJ
影响因子：
2.7
作者：
Itaya, M;Fujita, K;Tsuge, K
通讯作者：
Tsuge, K
影响因子：
3.2
作者：
Jakutyte, Lina;Baptista, Catarina;Tavares, Paulo
通讯作者：
Tavares, Paulo