Characterizing the Effect of Processing Technique and Solution Type on Cytomorphology Using Liquid-Based Cytology
Characterizing the Effect of Processing Technique and Solution Type on Cytomorphology Using Liquid-Based Cytology
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使用液基细胞学表征处理技术和溶液类型对细胞形态学的影响
DOI：
10.1159/000519335
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发表时间：
2021
期刊：
影响因子：
1.8
通讯作者：
Nagata Kohzo
中科院分区：
文献类型：
--
作者：
Ikeda Katsuhide;Oboshi Wataru;Hashimoto Yusuke;Komene Tetsuya;Yamaguchi Yoshitaka;Sato Shouichi;Maruyama Sayumi;Furukawa Nozomi;Sakabe Nanako;Nagata Kohzo
液基细胞学（LBC）越来越多地用于非妇科应用。然而，LBC标本的细胞学制备受到处理技术和所用防腐剂的影响。在这项研究中，加工技术和防腐剂对细胞形态的影响进行了数学和statistics.MethodsCytological标本制备使用的ThinPrep（TP），SurePath（SP），和AutoScrum方法，与5种不同的防腐剂解决方案。Papanicolaou染色标本的细胞质和细胞核面积进行了测量，为所有samples.ResultsThe细胞质和细胞核面积较小，使用2 LBC的方法制备的细胞，相比，使用AutoScrum方法制备的，无论使用的防腐剂。无论使用何种防腐剂，使用SP法制备的细胞的胞质和核面积均小于使用TP法制备的细胞。有较少的差异与不同的防腐剂解决方案，使用TP方法制备的细胞的胞质面积之间;然而，使用SP方法制备的细胞的胞质面积改变与防腐剂solutionused.ConclusionsThe最显着的差异影响细胞质和核面积的处理技术。TP方法增加了细胞质和细胞核面积，而基于甲醇的PreservCyt溶液能够使细胞最大化。LBC是一种用于标准化标本的上级制备技术。我们的研究结果提供了一个更好的理解的方法，适合标本制备开发精确的AI为基础的细胞学诊断。
IntroductionLiquid-based cytology (LBC) is increasingly used for nongynecologic applications. However, the cytological preparation of LBC specimens is influenced by the processing technique and the preservative used. In this study, the influence of the processing techniques and preservatives on cell morphology was examined mathematically and statistically.MethodsCytological specimens were prepared using the ThinPrep (TP), SurePath (SP), and AutoSmear methods, with 5 different preservative solutions. The cytoplasmic and nuclear areas of Papanicolaou-stained specimens were measured for all samples.ResultsThe cytoplasmic and nuclear areas were smaller in cells prepared using the 2 LBC methods, compared to that prepared using the AutoSmear method, irrespective of the preservative used. The cytoplasmic and nuclear areas of cells prepared using the SP method were smaller than those of cells prepared using the TP method, irrespective of the preservative used. There were fewer differences among the cytoplasmic areas of cells prepared with different preservative solutions using the TP method; however, the cytoplasmic areas of cells prepared using the SP method changed with the preservative solution used.ConclusionsThe most significant difference affecting the cytoplasmic and nuclear areas was the processing technique. The TP method increased the cytoplasmic and nuclear areas, while the methanol-based PreservCyt solution enabled the highest enlargement of the cell. LBC is a superior preparation technique for standardization of the specimens. Our results offer a better understanding of methods suitable for specimen preparation for developing precision AI-based diagnosis in cytology.
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使用阳性预测值、非典型预测值和总预测值作为性能指标比较 SurePath® 和 ThinPrep® 液基宫颈细胞学检查
影响因子：
1.3
作者：
P. K. Wright;J. Marshall;M. Desai
通讯作者：
M. Desai
影响因子：
1.3
作者：
Michael, CW;McConnel, J;Al-Khafaji, B
通讯作者：
Al-Khafaji, B
影响因子：
1.3
作者：
Belsley, Nicole A.;Tambouret, Rosemary H.;Wilbur, David C.
通讯作者：
Wilbur, David C.
液基细胞学：有效性、成本效益评估以及在当前实践中的应用。
DOI：
--
发表时间：
2004
期刊：
The Journal of the National Comprehensive Cancer Network
影响因子：
--
作者：
J. Thomas Cox
通讯作者：
J. Thomas Cox
影响因子：
1.3
作者：
Ikeda K;Tsuta K
通讯作者：
Tsuta K