Conserved genomic organisation of Group B Sox genes in insects.
Conserved genomic organisation of Group B Sox genes in insects.
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DOI：
10.1186/1471-2156-6-26
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发表时间：
2005-05-19
期刊：
影响因子：
2.9
通讯作者：
Russell S
中科院分区：
文献类型：
--
作者：
McKimmie C;Woerfel G;Russell S
含有 Sox 结构域的基因是重要的后生动物转录调节因子，与广泛的发育过程有关。脊椎动物 B 亚群包含在神经发育中具有早期功能的 Sox1、Sox2 和 Sox3 基因。先前的研究表明，果蝇 B 组基因在功能上是保守的，因为它们在早期神经规范中发挥着重要作用，并且果蝇二毛类和 SoxN 基因的突变可以用哺乳动物 Sox 基因来挽救。尽管 B 组家族很重要，但其在果蝇中的范围和组织尚未得到充分表征，这是利用果蝇检查 B 组 Sox 基因功能保守方面的重要一步。我们使用定向 cDNA 测序以及公开基因组测序项目的输出来检查果蝇、伪暗果蝇、冈比亚按蚊和蜜蜂中 B 组 Sox 结构域基因的结构。所有昆虫基因组都包含四个编码 B 组蛋白的基因，其中两个是无内含子的，就像脊椎动物 B 组基因的情况一样。正如之前报道的那样，B 组基因中的两个昆虫 B 组基因 Sox21a 和 Sox21b 在其 DNA 结合域内包含内含子。我们发现 Sox21b 基因的高度不寻常的多外显子结构对于昆虫来说是常见的。此外，我们发现 B 组 Sox 基因中的三个在昆虫基因组中组织成一个连锁簇。通过原位杂交，我们发现胚胎发生过程中四个 B 组基因中每一个的表达模式在黑腹果蝇和假暗果蝇之间是保守的。自膜翅目和双翅目最后一个共同祖先以来，B 组基因的 DNA 结合结构域序列和基因组组织已经保守了 300 多年的进化。我们的分析表明昆虫有两个 B1 组基因，SoxN 和 Dichaete，以及两个 B2 组基因。 Dichaete 和另外两个 B 组基因在一个簇中的基因组组织表明，它们可能受到协调一致的监管控制。我们的分析提出了昆虫 B 组 Sox 基因进化的简单模型，该模型不同于脊椎动物 B 组基因的拟议进化。
Sox domain containing genes are important metazoan transcriptional regulators implicated in a wide rage of developmental processes. The vertebrate B subgroup contains the Sox1, Sox2 and Sox3 genes that have early functions in neural development. Previous studies show that Drosophila Group B genes have been functionally conserved since they play essential roles in early neural specification and mutations in the Drosophila Dichaete and SoxN genes can be rescued with mammalian Sox genes. Despite their importance, the extent and organisation of the Group B family in Drosophila has not been fully characterised, an important step in using Drosophila to examine conserved aspects of Group B Sox gene function. We have used the directed cDNA sequencing along with the output from the publicly-available genome sequencing projects to examine the structure of Group B Sox domain genes in Drosophila melanogaster, Drosophila pseudoobscura, Anopheles gambiae and Apis mellifora. All of the insect genomes contain four genes encoding Group B proteins, two of which are intronless, as is the case with vertebrate group B genes. As has been previously reported and unusually for Group B genes, two of the insect group B genes, Sox21a and Sox21b, contain introns within their DNA-binding domains. We find that the highly unusual multi-exon structure of the Sox21b gene is common to the insects. In addition, we find that three of the group B Sox genes are organised in a linked cluster in the insect genomes. By in situ hybridisation we show that the pattern of expression of each of the four group B genes during embryogenesis is conserved between D. melanogaster and D. pseudoobscura. The DNA-binding domain sequences and genomic organisation of the group B genes have been conserved over 300 My of evolution since the last common ancestor of the Hymenoptera and the Diptera. Our analysis suggests insects have two Group B1 genes, SoxN and Dichaete, and two Group B2 genes. The genomic organisation of Dichaete and another two Group B genes in a cluster, suggests they may be under concerted regulatory control. Our analysis suggests a simple model for the evolution of group B Sox genes in insects that differs from the proposed evolution of vertebrate Group B genes.
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DOI：
10.1073/pnas.231608898
发表时间：
2002-01-22
影响因子：
11.1
作者：
Berman, BP;Nibu, Y;Eisen, MB
通讯作者：
Eisen, MB
DOI：
10.1159/000015149
发表时间：
1998-01-01
期刊：
CYTOGENETICS AND CELL GENETICS
影响因子：
--
作者：
Arsic, N;Rajic, T;Stevanovic, M
通讯作者：
Stevanovic, M
影响因子：
2.6
作者：
Crémazy, F;Berta, P;Girard, F
通讯作者：
Girard, F
影响因子：
14.9
作者：
Drysdale RA;Crosby MA;FlyBase Consortium
通讯作者：
FlyBase Consortium
影响因子：
14.9
作者：
Chenna, R;Sugawara, H;Thompson, JD
通讯作者：
Thompson, JD