Particle focusing in staged inertial microfluidic devices for flow cytometry.-猫眼课题宝

DOI：

10.1021/ac100387b

发表时间：

2010-05-01

期刊：

ANALYTICAL CHEMISTRY

影响因子：

7.4

通讯作者：

中科院分区：

化学1区

文献类型：

作者：

Oakey, John;Applegate, Robert W., Jr.;Arellano, Erik;Di Carlo, Dino;Graves, Steven W.;Toner, Mehmet

来源链接：

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3136802/?report=classic

微流控惯性聚焦已被证明是一种有效的无鞘液辅助的微粒子和细胞被动定位方法。由于惯性聚焦产生明确的横向平衡粒子位置以及高度调节的粒子间距，它在流式细胞术中的价值已被提出。粒子聚焦发生在直线通道中，并且可以通过引入曲率的横截面通道几何来操纵。在这里，我们提出了一个分阶段的通道设计，包括弯曲和直线部分，结合有序粒子成一个纵向间隔的单一流线。我们使用使用校准微球的标准流式细胞术方法评估了这些阶段惯性聚焦通道的性能。我们的分析确定了测量精度和分辨率，作为这些通道提供的流速和颗粒浓度的函数。在检测范围内，这些设备在更高的流速和颗粒浓度下具有更高的效率，是高通量分析的理想选择。此外，配备惯性聚焦微通道的原型流式细胞仪与商用流体动力聚焦流式细胞仪提供的分辨率相匹配。最值得注意的是，我们的分析表明，惯性聚焦通道实际上消除了分析点的粒子重合。这些特性表明惯性聚焦在发展廉价的基于流式细胞术的诊断和需要分析高浓度颗粒的应用中具有潜在的重要作用。

Microfluidic inertial focusing has been demonstrated to be an effective method for passively positioning microparticles and cells without the assistance of sheath fluid. Because inertial focusing produces well-defined lateral equilibrium particle positions in addition to highly regulated interparticle spacing, its value in flow cytometry has been suggested. Particle focusing occurs in straight channels and can be manipulated through cross sectional channel geometry by the introduction of curvature. Here, we present a staged channel design consisting of both curved and straight sections that combine to order particles into a single streamline with longitudinal spacing. We have evaluated the performance of these staged inertial focusing channels using standard flow cytometry methods that make use of calibration microspheres. Our analysis has determined the measurement precision and resolution, as a function of flow velocity and particle concentration that is provided by these channels. These devices were found to operate with increasing effectiveness at higher flow rates and particle concentrations, within the examined ranges, which is ideal for high throughput analysis. Further, the prototype flow cytometer equipped with an inertial focusing microchannel matched the resolution provided by a commercial hydrodynamic focusing flow cytometer. Most notably, our analysis indicates that the inertial focusing channels virtually eliminated particle coincidence at the analysis point. These properties suggest a potentially significant role for inertial focusing in the development of inexpensive flow cytometry-based diagnostics and in applications requiring the analysis of high particle concentrations.