Tumor necrosis factor alpha-mediated tumor regression by the in vivo transfer of genes into the artery that leads to tumors.
Tumor necrosis factor alpha-mediated tumor regression by the in vivo transfer of genes into the artery that leads to tumors.
复制标题
肿瘤坏死因子α通过将基因体内转移到导致肿瘤的动脉中来介导肿瘤消退。
作者：
H. Mizuguchi;T. Nakagawa;S. Toyosawa;M. Nakanishi;S. Imazu;T. Nakanishi;Y. Tsutsumi;S. Nakagawa;T. Hayakawa;N. Ijuhin;T. Mayumi
我们报道，肿瘤坏死因子(TNF) α在体内通过基因转移在动脉中产生导致肿瘤时，诱导了强烈的抗肿瘤免疫反应。我们用右足部携带肉瘤-180瘤的小鼠模型，将包裹人tnf - α基因的融合性脂质体注入右股动脉。在这种条件下，人类tnf - α仅在通向肿瘤的动脉和肿瘤中检测到。将tnf - α基因注入右股动脉后，肿瘤生长明显消退，11只小鼠中有4只完全治愈。当tnf - α基因进入左股动脉或肿瘤时，或当给予荧光素酶基因时，均未观察到倒退。注射抗tnf - α、抗cd4或抗CD8单克隆抗体可抑制肿瘤消退，tnf - α处理小鼠肿瘤中CD8+ T细胞积累。这些结果表明，在导致肿瘤的动脉中局部表达的tnf - α通过增强抗肿瘤免疫反应有效地抑制肿瘤生长。讨论了这一现象对肿瘤基因治疗的意义。
We report that tumor necrosis factor (TNF) alpha induced a strong antitumor immune reaction when it was produced in arteries leading to tumors by gene transfer in vivo. We used a mouse model carrying a sarcoma-180 tumor in the right footpad and injected the fusogenic liposomes encapsulating the human TNF-alpha gene into the right femoral artery. Under this condition, human TNF-alpha was detected only in the artery leading to the tumor and in the tumor. There was a significant regression in tumor growth when the TNF-alpha gene was delivered into the right femoral artery, with 4 of 11 mice completely cured. No regression was observed when the TNF-alpha gene was delivered into the left femoral artery or into the tumor or when the luciferase gene was administered. Tumor regression was inhibited by the injection of anti-TNF-alpha, anti-CD4, or anti-CD8 monoclonal antibody, and CD8+ T cells accumulated in the tumors of TNF-alpha-treated mice. These results suggest that TNF-alpha expressed locally in the arteries leading to tumors efficiently suppresses tumor growth through reinforcement of an antitumor immune reaction. The significance of this phenomenon for cancer gene therapy was discussed.
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通过体内基因转移到肿瘤中进行恶性肿瘤的免疫治疗。
DOI：
10.1073/pnas.90.10.4645
发表时间：
1993
影响因子：
11.1
作者：
Plautz,GE;Yang,ZY;Wu,BY;Gao,X;Huang,L;Nabel,GJ
通讯作者：
Nabel,GJ
影响因子：
4.4
作者：
M. Sarmiento;A. Glasebrook;F. Fitch
通讯作者：
M. Sarmiento;A. Glasebrook;F. Fitch
通过单克隆抗体 GK1.5 鉴定的鼠 T 细胞表面分子（称为 L3T4）的特征：L3T4 与人 Leu-3/T4 分子的相似性。
DOI：
--
发表时间：
1983
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Dialynas,DP;Quan,ZS;Wall,KA;Pierres,A;Quintans,J;Loken,MR;Pierres,M;Fitch,FW
通讯作者：
Fitch,FW
肿瘤坏死因子-α 增强人类自然杀伤细胞的溶细胞活性。
DOI：
--
发表时间：
1987
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Ostensen,ME;Thiele,DL;Lipsky,PE
通讯作者：
Lipsky,PE
DOI：
10.1073/pnas.84.21.7413
发表时间：
1987-11-01
影响因子：
11.1
作者：
FELGNER, PL;GADEK, TR;DANIELSEN, M
通讯作者：
DANIELSEN, M