In vitro labeling of endothelial progenitor cells isolated from peripheral blood with superparamagnetic iron oxide nanoparticles.
In vitro labeling of endothelial progenitor cells isolated from peripheral blood with superparamagnetic iron oxide nanoparticles.
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用超顺磁性氧化铁纳米粒子体外标记从外周血中分离的内皮祖细胞
DOI：
10.3892/mmr.2012.912
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发表时间：
2012-08
影响因子：
3.4
通讯作者：
Zheng SS
中科院分区：
文献类型：
--
作者：
Sun JH;Zhang YL;Nie CH;Qian SP;Yu XB;Xie HY;Zhou L;Zheng SS
The transplantation of endothelial progenitor cells (EPCs) provides a novel method for the treatment of human tumors or vascular diseases. Magnetic resonance imaging (MRI) has proven to be effective in tracking transplanted stem cells by labeling the cells with superparamagnetic iron oxide (SPIO) nanoparticles. The SPIO has been used to label and track the EPCs; however, the effect of SPIO upon EPCs remains unclear on a cellular level. In the present study, EPCs were labeled with home-synthesized SPIO nanoparticles in vitro and the biological characteristics of the labeled EPCs were evaluated. The EPCs were isolated from the peripheral blood of New Zealand rabbits and cultured in fibronectin-coated culture flasks. The EPCs were labeled with home-synthesized SPIO nanoparticles at a final iron concentration of 20 μg/ml. Labeled EPCs were confirmed with transmission electron microscopy and Prussian blue staining. The quantity of iron/cell was detected by atomic absorption spectrometry. The membranous antigens of EPCs were detected by cytofluorimetric analysis. Cell viability and proliferative capability between the labeled and unlabeled EPCs were compared. The rabbit EPCs were effectively labeled and the labeling efficiency was approximately 95%. The SPIO nanoparticles were localized in the endosomal vesicles of the EPCs, which were confirmed by transmission electron microscopy. No significant differences were found in cell viability and proliferative capability between labeled and unlabeled EPCs (P>0.05). In conclusion, rabbit peripheral blood EPCs were effectively labeled by home-synthesized SPIO nanoparticles, without influencing their main biological characteristics.
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影响因子：
56.9
作者：
Asahara, T;Murohara, T;Isner, JM
通讯作者：
Isner, JM
影响因子：
9.8
作者：
Liang, Paulina H.;Tian, Fang;Lu, Yi;Duan, Biyan;Stolz, Donna B.;Li, Lu-Yuan
通讯作者：
Li, Lu-Yuan
影响因子：
20.3
作者：
Sato, Y;Araki, H;Niitsu, Y
通讯作者：
Niitsu, Y
影响因子：
11.5
作者：
Hung, SC;Deng, WP;Gelovani, JG
通讯作者：
Gelovani, JG
影响因子：
8.3
作者：
Jickling, Glen;Salam, Abdul;Camicioli, Richard
通讯作者：
Camicioli, Richard