Structural Contributions to Hydrodynamic Diameter for Quantum Dots Optimized for Live-Cell Single-Molecule Tracking.
Structural Contributions to Hydrodynamic Diameter for Quantum Dots Optimized for Live-Cell Single-Molecule Tracking.
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针对活细胞单分子跟踪优化的量子点的流体动力学直径的结构贡献。
DOI：
10.1021/acs.jpcc.8b02516
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Selvin,PaulR
中科院分区：
文献类型：
--
作者：
Sheung,JanetY;Ge,Pinghua;Lim,SungJun;Lee,SangHak;Smith,AndrewM;Selvin,PaulR
量子点是具有窄带、尺寸可调和持久发射的荧光纳米颗粒。用于细胞中蛋白质成像的典型制剂在流体动力学上比蛋白质靶大得多，因此评估源自流体动力学尺寸的空间效应的影响至关重要。这份报告分析了一类新的量子点，这些量子点已经被设计成最小化的尺寸，专门用于神经元之间狭窄的突触连接中的成像受体。我们使用荧光相关光谱和透射电子显微镜计算的贡献的结晶核心，有机涂层，和靶向蛋白质（链霉亲和素）的总流体动力学直径的探针，使用范围广泛的核心材料与发射跨越545-705 nm。我们发现标准商业两亲聚合物的贡献厚度为0.8nm至0.14nm，而基于紧凑配体HS-（CH 2）11-（OCH 2CH 2）4-OH的涂层贡献0.6nm至0.99nm，根据核心尺寸，直径减小0.2nm至0.5nm。当用于蛋白质靶向的链霉亲和素的数量最小化时，总直径可以进一步减小0.5至0.11 nm，产生13.8-18.4 nm的直径。这些发现解释了为什么进入狭窄的突触主要来自商业变体的蛋白质功能化，而不是有机涂层。他们还解释了为什么那些只有少量链霉亲和素的尺寸约为14 nm的量子点可以进入狭窄的细胞结构进行神经元标记，而那些>27 nm和大量链霉亲和素的量子点则不能。
Quantum dots are fluorescent nanoparticles with narrow-band, size-tunable, and long-lasting emission. Typical formulations used for imaging proteins in cells are hydrodynamically much larger than the protein targets, so it is critical to assess the impact of steric effects deriving from hydrodynamic size. This report analyzes a new class of quantum dots that have been engineered for minimized size specifically for imaging receptors in narrow synaptic junctions between neurons. We use fluorescence correlation spectroscopy and transmission electron microscopy to calculate the contributions of the crystalline core, organic coating, and targeting proteins (streptavidin) to the total hydrodynamic diameter of the probe, using a wide range of core materials with emission spanning 545–705 nm. We find the contributing thickness of standard commercial amphiphilic polymers to be ∼8 to ∼14 nm, whereas coatings based on the compact ligand HS-(CH2)11-(OCH2CH2)4-OH contribute ∼6 to ∼9 nm, reducing the diameter by ∼2 to ∼5 nm, depending on core size. When the number of streptavidins for protein targeting is minimized, the total diameter can be further reduced by ∼5 to ∼11 nm, yielding a diameter of 13.8–18.4 nm. These findings explain why access to the narrow synapse derive primarily from the protein functionalization of commercial variants, rather than the organic coating layers. They also explain why those quantum dots with size around 14 nm with only a few streptavidins can access narrow cellular structures for neuronal labeling, whereas those >27 nm and a large number of streptavidins, cannot.
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影响因子：
15
作者：
Ma, Liang;Tu, Chunlai;Smith, Andrew M.
通讯作者：
Smith, Andrew M.
DOI：
10.1073/pnas.97.1.151
发表时间：
2000-01-04
影响因子：
11.1
作者：
Schwille, P;Kummer, S;Webb, WW
通讯作者：
Webb, WW
影响因子：
3.3
作者：
de Thomaz, A. A.;Almeida, D. B.;Cesar, C. L.
通讯作者：
Cesar, C. L.
使用不同尺寸荧光探针对 AMPA 受体突触和突触外池进行超分辨率成像
DOI：
10.1101/096966
发表时间：
2016
期刊：
bioRxiv
影响因子：
--
作者：
Sang Hak Lee;Chaoyi Jin;En Cai;Pinghua Ge;Y. Ishitsuka;K. Teng;A. Thomaz;Duncan L Nall;M. Baday;Okunola Jeyifous;D. Demonte;Christopher M. Dundas;Sheldon Park;W. N. Green;P. Selvin
通讯作者：
P. Selvin
影响因子：
16.6
作者：
Cai, En;Ge, Pinghua;Lee, Sang Hak;Jeyifous, Okunola;Wang, Yong;Liu, Yanxin;Wilson, Katie M.;Lim, Sung Jun;Baird, Michelle A.;Stone, John E.;Lee, Kwan Young;Davidson, Michael W.;Chung, Hee Jung;Schulten, Klaus;Smith, Andrew M.;Green, William N.;Selvin, Paul R.
通讯作者：
Selvin, Paul R.