CLASPs link focal-adhesion-associated microtubule capture to localized exocytosis and adhesion site turnover.
CLASPs link focal-adhesion-associated microtubule capture to localized exocytosis and adhesion site turnover.
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DOI：
10.1038/ncb2975
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发表时间：
2014-06
影响因子：
21.3
通讯作者：
中科院分区：
文献类型：
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作者：
整合素为基础的局灶黏附（FAs）与细胞外基质（ECM）的转换是协调细胞运动所必需的。在集体迁移的人角质形成细胞中，FAs聚集在前缘附近，由于收缩力而生长和成熟，并在前进的细胞体下分解。我们报道，微管相关的CLASP1和CLASP2蛋白聚集在FAs周围与FA周转有时间相关性。class和LL5β（将class招募到FAs）促进FA的分解。FA相关的ECM降解进一步需要CLASPs，基质金属蛋白酶抑制减缓FA分解，类似于CLASP或LL5β耗竭。最后，clasp介导的FAs微管系聚建立了一个fa导向的转运途径，用于FAs附近的胞外囊泡的递送、对接和局部融合。我们认为，CLASPs结合了微管组织、囊泡运输和细胞与ECM的相互作用，建立了一个局部分泌途径，通过切断细胞-基质连接促进FA的周转。
Turnover of integrin-based focal adhesions (FAs) with the extracellular matrix (ECM) is essential for coordinated cell movement. In collectively migrating human keratinocytes, FAs assemble near the leading edge, grow and mature as a result of contractile forces, and disassemble underneath the advancing cell body. We report that clustering of microtubule-associated CLASP1 and CLASP2 proteins around FAs temporally correlates with FA turnover. CLASPs and LL5β, which recruits CLASPs to FAs, facilitate FA disassembly. CLASPs are further required for FA-associated ECM degradation, and matrix metalloprotease inhibition slows FA disassembly similar to CLASP or LL5β depletion. Finally, CLASP-mediated microtubuletethering at FAs establishes a FA-directed transport pathway for delivery, docking and localized fusion of exocytic vesicles near FAs. We propose that CLASPs couple microtubule organization, vesicle transport and cell interactions with the ECM, establishing a local secretion pathway that facilitates FA turnover by severing cell-matrix connections.
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影响因子：
9.2
作者：
Gierke, Sarah;Wittmann, Torsten
通讯作者：
Wittmann, Torsten
影响因子：
11.4
作者：
Bravo-Cordero, Jose J.;Marrero-Diaz, Raquel;Montoya, Maria C.
通讯作者：
Montoya, Maria C.
影响因子：
56.9
作者：
Hu, Ke;Ji, Lin;Waterman-Storer, Clare M.
通讯作者：
Waterman-Storer, Clare M.
影响因子：
11.8
作者：
Grigoriev, Ilya;Splinter, Daniel;Akhmanova, Anna
通讯作者：
Akhmanova, Anna
DOI：
10.1083/jcb.200904054
发表时间：
2009-11-30
期刊：
The Journal of cell biology
影响因子：
--
作者：
Ezratty EJ;Bertaux C;Marcantonio EE;Gundersen GG
通讯作者：
Gundersen GG