Bacterial communities in pigmented biofilms formed on the sandstone bas-relief walls of the Bayon Temple, Angkor Thom, Cambodia.
Bacterial communities in pigmented biofilms formed on the sandstone bas-relief walls of the Bayon Temple, Angkor Thom, Cambodia.
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DOI：
10.1264/jsme2.me13033
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发表时间：
2013
影响因子：
2.2
通讯作者：
Katayama Y
中科院分区：
文献类型：
--
作者：
Kusumi A;Li X;Osuga Y;Kawashima A;Gu JD;Nasu M;Katayama Y
柬埔寨吴哥窟的巴戎寺已经严重退化，并形成了各种色素生物膜。由于生物膜破坏了砂岩表面的浅浮雕，浅浮雕，关于其中微生物群落的信息对于控制生物膜定殖是必不可少的。从有色砂岩表面的生物膜样品的PCR-变性梯度凝胶电泳（DGGE）分析表明，在生物膜中的细菌群落成员明显不同于那些在空气中，并具有较低的序列相似性数据库序列。生物膜的非破坏性取样揭示了新的细菌群，主要是红杆菌属在橙红色生物膜中，蓝细菌属在Chrome绿色生物膜中，蓝细菌属和绿球藻属在信号紫色生物膜中，绿球藻属在黑灰色生物膜中，以及异常球菌属-栖热菌属、蓝细菌属和红杆菌属在蓝色绿色生物膜中。连续剥离的厚生物膜层与胶粘片揭示了分层结构：蓝绿色的生物膜，周围有严重的恶化，是非常丰富的蓝藻靠近表面和Chloroflexi在下面的深层。蓝绿色生物膜中硝酸根离子浓度较高。细菌在不同生物膜深度的分布特征不仅为生物膜的形成过程提供了有价值的信息，也为热带地区砂岩的风化过程提供了有价值的信息。
The Bayon temple in Angkor Thom, Cambodia has shown serious deterioration and is subject to the formation of various pigmented biofilms. Because biofilms are damaging the bas-reliefs, low reliefs engraved on the surface of sandstone, information about the microbial community within them is indispensable to control biofilm colonization. PCR-denaturing gradient gel electrophoresis (DGGE) analysis of biofilm samples from the pigmented sandstone surfaces showed that the bacterial community members in the biofilms differed clearly from those in the air and had low sequence similarity to database sequences. Non-destructive sampling of biofilm revealed novel bacterial groups of predominantly Rubrobacter in salmon pink biofilm, Cyanobacteria in chrome green biofilm, Cyanobacteria and Chloroflexi in signal violet biofilm, Chloroflexi in black gray biofilm, and Deinococcus-Thermus, Cyanobacteria, and Rubrobacter in blue green biofilm. Serial peeling-off of a thick biofilm by layers with adhesive sheets revealed a stratified structure: the blue–green biofilm, around which there was serious deterioration, was very rich in Cyanobacteria near the surface and Chloroflexi in deep layer below. Nitrate ion concentrations were high in the blue–green biofilm. The characteristic distribution of bacteria at different biofilm depths provides valuable information on not only the biofilm formation process but also the sandstone weathering process in the tropics.
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影响因子：
5.2
作者：
Kusumi A;Li XS;Katayama Y
通讯作者：
Katayama Y
影响因子：
2.2
作者：
Li, Xianshu;Arai, Hideo;Katayama, Yoko
通讯作者：
Katayama, Yoko
DOI：
10.1099/00207713-50-4-1529
发表时间：
2000-07-01
影响因子：
2.8
作者：
Keppen, OI;Tourova, TP;Gorlenko, VM
通讯作者：
Gorlenko, VM
影响因子：
--
作者：
Laiz, L.;Miller, A. Z.;Saiz-Jimenez, C.
通讯作者：
Saiz-Jimenez, C.
影响因子：
4.4
作者：
Cole, AC;Semmens, MJ;LaPara, TM
通讯作者：
LaPara, TM