Conditional Knockout of Bmal1 in Corticotropin-Releasing Factor Neurons Does Not Alter Sleep-Wake Rhythm in Mice.
Conditional Knockout of Bmal1 in Corticotropin-Releasing Factor Neurons Does Not Alter Sleep-Wake Rhythm in Mice.
复制标题
条件敲除促肾上腺皮质激素释放因子神经元中的Bmal1不会改变小鼠的睡眠-觉醒节律。
DOI：
10.3389/fnins.2021.808754
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发表时间：
2021
影响因子：
4.3
通讯作者：
Ono D
中科院分区：
文献类型：
--
作者：
Hung CJ;Yamanaka A;Ono D
睡眠和清醒都受体内平衡机制和生物钟的调节。在哺乳动物中，中央生物钟，即下丘脑中的视交叉上核，在生理和行为的时间安排中起着至关重要的作用。最近，我们发现觉醒的昼夜调节是通过下丘脑室旁核中的促肾上腺皮质激素释放因子(CRF)神经元传递到下丘脑外侧的增食欲素神经元。然而，目前还不清楚CRF神经元中的分子时钟如何参与睡眠和清醒的调节。在本研究中，我们建立了CRF神经元特异性BMal1缺陷小鼠，并测量了运动活动或脑电和肌电。我们发现，这些小鼠在明暗循环和持续黑暗中都表现出正常的昼夜运动活动节律。此外，他们表现出正常的睡眠和清醒的日常模式。这些结果表明，CRF神经元中的BMal1对昼夜运动活动或睡眠和觉醒都没有影响。
Sleep and wakefulness are regulated by both the homeostatic mechanism and circadian clock. In mammals, the central circadian clock, the suprachiasmatic nucleus, in the hypothalamus plays a crucial role in the timing of physiology and behavior. Recently, we found that the circadian regulation of wakefulness was transmitted via corticotropin-releasing factor (CRF) neurons in the paraventricular nucleus of the hypothalamus to orexin neurons in the lateral hypothalamus. However, it is still unclear how the molecular clock in the CRF neurons contributes to the regulation of sleep and wakefulness. In the present study, we established CRF neuron-specific Bmal1-deficient mice and measured locomotor activity or electroencephalography and electromyography. We found that these mice showed normal circadian locomotor activity rhythms in both light–dark cycle and constant darkness. Furthermore, they showed normal daily patterns of sleep and wakefulness. These results suggest that Bmal1 in CRF neurons has no effect on either circadian locomotor activity or sleep and wakefulness.
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影响因子：
13.6
作者：
Ono D;Mukai Y;Hung CJ;Chowdhury S;Sugiyama T;Yamanaka A
通讯作者：
Yamanaka A
影响因子：
13.6
作者：
Li, Shi-Bin;Borniger, Jeremy C.;de Lecea, Luis
通讯作者：
de Lecea, Luis
影响因子：
2.9
作者：
SAWAKI Y;NIHONMATSU I;KAWAMURA H
通讯作者：
KAWAMURA H
影响因子：
64.8
作者：
Ciocchi, Stephane;Herry, Cyril;Luethi, Andreas
通讯作者：
Luethi, Andreas
影响因子：
64.8
作者：
Gizowski, C.;Zaelzer, C.;Bourque, C. W.
通讯作者：
Bourque, C. W.