Transcriptome analysis of duck embryo fibroblasts for the dynamic response to duck tembusu virus infection and dual regulation of apoptosis genes.
Transcriptome analysis of duck embryo fibroblasts for the dynamic response to duck tembusu virus infection and dual regulation of apoptosis genes.
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鸭胚胎成纤维细胞对鸭坦布苏病毒感染的动态反应和凋亡基因双重调控的转录组分析
DOI：
10.18632/aging.103759
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发表时间：
2020-09-07
期刊：
影响因子：
--
通讯作者：
Jia R
中科院分区：
文献类型：
--
作者：
Pan Y;Cheng A;Zhang X;Wang M;Chen S;Zhu D;Liu M;Zhao X;Yang Q;Wu Y;Huang J;Zhang S;Mao S;Ou X;Gao Q;Yu Y;Liu Y;Zhang L;Yin Z;Jing B;Tian B;Pan L;Rehman MU;Chen X;Jia R
鸭Tembusu病毒（DTMUV）是一种新兴的致病性黄病毒，在东南亚造成了巨大的经济损失。然而，DTMUV感染后的致病机制和宿主反应仍然知之甚少。在本研究中，采用总mRNA测序（RNA-Seq）分析方法检测DTMUV感染后不同时间点DEFs中基因的整体表达。我们发现326个基因在所有时间点都发生了显著变化，这些基因在多种生物过程中动态富集，包括细胞凋亡、先天免疫反应、DNA复制、细胞周期阻滞和DNA修复。结果表明：凋亡被诱导，凋亡比例随时间增加，促凋亡分子caspase被激活。RNA-seq数据分析进一步显示，大多数促凋亡和抗凋亡基因早期持续响应，并且参与内源性和外源性凋亡途径的基因都被启动。此外，大量丰富的免疫相关通路参与凋亡过程，蛋白-蛋白相互作用（PPIs）分析显示，IL6、STAT1、TNFAIP3、CFLAR和PTGS2可能是DEFs凋亡的关键调控因子。综上所述，本研究不仅有助于了解DEFs感染DTMUV的潜在机制，而且为抗病毒治疗的靶点筛选提供了新的见解。
Duck Tembusu virus (DTMUV) is an emerging pathogenic flavivirus that has caused enormous economic losses in Southeast Asia. However, the pathogenic mechanism and host’s responses after DTMUV infection remain poorly understood. During this study, total mRNA sequencing (RNA-Seq) analysis was used to detect the global gene expression in DEFs at various time points after DTMUV infection. We identified 326 genes altered significantly at all time points, and these genes were dynamically enriched in multifarious biological processes, including apoptosis, innate immune response, DNA replication, cell cycle arrest and DNA repair. Next, the results showed that apoptosis was induced and the proportion of apoptosis increased with time, and pro-apoptotic molecules caspases were activated. The RNA-seq data analysis further revealed that most pro-apoptosis and anti-apoptosis genes were early continually responsive, and the genes involved in both intrinsic and extrinsic apoptotic pathways were initiated. Further, the considerably enriched immune-relevant pathways were involved in apoptosis process, and protein-protein interactions (PPIs) analysis showed that IL6, STAT1, TNFAIP3, CFLAR and PTGS2 may be key regulators of DEFs apoptosis. In conclusion, this study not only contributes to understanding the underlying mechanism of DEFs infection with DTMUV, but also provides new insights into targets screening for antiviral therapy.
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