The effect of emodin, an anthraquinone derivative extracted from the roots of Rheum tanguticum, against herpes simplex virus in vitro and in vivo.
The effect of emodin, an anthraquinone derivative extracted from the roots of Rheum tanguticum, against herpes simplex virus in vitro and in vivo.
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唐古特大黄根中提取的蒽醌衍生物大黄素体内外抗单纯疱疹病毒的作用
DOI：
10.1016/j.jep.2010.10.059
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发表时间：
2011-01-27
影响因子：
5.4
通讯作者：
Yang ZQ
中科院分区：
文献类型：
--
作者：
Xiong HR;Luo J;Hou W;Xiao H;Yang ZQ
单纯疱疹病毒（HSV-1和HSV-2）是人类重要的病原体，寻找新型低毒的抗HSV药物值得努力。大黄是最古老、最著名的传统中药之一。本研究旨在验证大黄素是否为唐古特大黄的有效成分。唐古特大黄（中国大黄之一）抗单纯疱疹病毒感染，并在组织培养细胞和小鼠模型中研究其对单纯疱疹病毒感染的抗病毒活性。大黄素（3-甲基-1，6，8-三羟基蒽醌）是从大黄中提取、纯化得到的一种有效成分。采用高效液相色谱法对青海高山地区栽培的唐古特甘草进行了纯度测定。对大黄素进行了体内外抗HSV感染实验。在体内，HSV感染的小鼠在HSV暴露后24小时开始口服大黄素，剂量分别为3.3 g/kg/天、6.7 g/kg/天和11.3 g/kg/天，持续7天。大黄素在50 μg/ml浓度下对HSV-1和HSV-2的增殖有抑制作用，抗病毒指数分别为2.07和3.53。与病毒对照组相比，大黄素治疗增加了HSV感染小鼠的存活率，延长了存活时间，并显示出更高的从脑、心脏、肝脏和神经节清除HSV的功效。此外，大黄素的体内抗病毒活性与阿昔洛韦相当。结果表明，大黄素在体内外均具有抗HSV活性，有望成为临床治疗HSV感染的有效药物。
Herpes simplex viruses (HSV-1 and -2) are important pathogens for humans and the discovery of novel anti-HSV drugs with low toxicity deserves great efforts. Rhubarb is one of the oldest and best-known traditional Chinese medicines. We initiated this study to test if emodin is the active ingredients from Rheum tanguticum (R. tanguticum, one of the Chinese Rhubarb) against HSV infection and to investigate its antiviral activity on HSV infection in tissue culture cells and in a mouse model. Emodin (3-methyl-1,6,8-trihydroxyanthraquinone) was extracted and purified from R. tanguticum (cultivated at high mountainous area in Qinghai) and the purity was determined by high performance liquid chromatography. The antiviral experiments of emodin against HSV infection were performed in vitro and in vivo. In vivo, the HSV-infected mice were orally administered with emodin beginning at 24 h post-HSV exposures with dosages of 3.3 g/kg/day, 6.7 g/kg/day, and 11.3 g/kg/day, respectively, for 7 days. Emodin was found to inhibit the replication of HSV-1 and HSV-2 in cell culture at the concentration of 50 μg/ml with antiviral index of 2.07 and 3.53, respectively. The emodin treatment increased the survival rate of HSV-infected mice, prolonged survival time and showed higher efficacy of HSV elimination from brain, heart, liver and ganglion, compared to the viral controls. In addition, the antiviral activity of emodin was found to be equivalent to that of acyclovir in vivo. Our results indicate that emodin has the anti-HSV activity in vitro and in vivo and is thus a promising agent in the clinical therapy of HSV infection.
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影响因子：
3.7
作者：
Abu-Raddad LJ;Magaret AS;Celum C;Wald A;Longini IM Jr;Self SG;Corey L
通讯作者：
Corey L
影响因子：
7.6
作者：
Ho TY;Wu SL;Chen JC;Li CC;Hsiang CY
通讯作者：
Hsiang CY
DOI：
10.1073/pnas.85.14.5230
发表时间：
1988-07-01
影响因子：
11.1
作者：
MERUELO, D;LAVIE, G;LAVIE, D
通讯作者：
LAVIE, D
影响因子：
2.4
作者：
Patel, Asha R;Romanelli, Paolo;Kirsner, Robert S
通讯作者：
Kirsner, Robert S
影响因子：
3.6
作者：
Mayaud, P.;Nagot, N.;Van de Perre, P
通讯作者：
Van de Perre, P