Visualization of abscess formation in a murine thigh infection model of Staphylococcus aureus by 19F-magnetic resonance imaging (MRI).
Visualization of abscess formation in a murine thigh infection model of Staphylococcus aureus by 19F-magnetic resonance imaging (MRI).
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DOI：
10.1371/journal.pone.0018246
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发表时间：
2011-03-24
期刊：
影响因子：
3.7
通讯作者：
Jakob P
中科院分区：
文献类型：
--
作者：
Hertlein T;Sturm V;Kircher S;Basse-Lüsebrink T;Haddad D;Ohlsen K;Jakob P
在过去的几年里，19F-MRI和全氟碳纳米乳（PFC）作为一种强大的造影剂为基础的MRI方法来跟踪细胞和可视化炎症。我们应用这种新模式来观察由金黄色葡萄球菌感染引起的急性和慢性炎症期的深部组织脓肿。在本研究中，采用小鼠大腿感染模型诱导脓肿形成，并在急性或慢性炎症期给予PFC或CLIO（交联氧化铁）。接种24 h后，MRI检查感染部位造影剂积累情况。在感染的急性和慢性阶段，测量显示脓肿边缘有很强的PFC积累。其形态与慢性期CLIO堆积相似，在水肿区周围形成空心球体。组织学显示感染部位有大量中性粒细胞内流，急性期有少量巨噬细胞内流，炎症慢性期有大量巨噬细胞内流。我们引入19F-MRI结合PFC纳米乳剂作为一个新的平台来观察金黄色葡萄球菌小鼠大腿感染模型的脓肿形成。通过这种方式跟踪体内免疫细胞的可能性为研究宿主免疫反应、抗菌治疗的疗效和毒力因素对发病机制的影响提供了新的机会。
During the last years, 19F-MRI and perfluorocarbon nanoemulsion (PFC) emerged as a powerful contrast agent based MRI methodology to track cells and to visualize inflammation. We applied this new modality to visualize deep tissue abscesses during acute and chronic phase of inflammation caused by Staphylococcus aureus infection. In this study, a murine thigh infection model was used to induce abscess formation and PFC or CLIO (cross linked ironoxides) was administered during acute or chronic phase of inflammation. 24 h after inoculation, the contrast agent accumulation was imaged at the site of infection by MRI. Measurements revealed a strong accumulation of PFC at the abscess rim at acute and chronic phase of infection. The pattern was similar to CLIO accumulation at chronic phase and formed a hollow sphere around the edema area. Histology revealed strong influx of neutrophils at the site of infection and to a smaller extend macrophages during acute phase and strong influx of macrophages at chronic phase of inflammation. We introduce 19F-MRI in combination with PFC nanoemulsions as a new platform to visualize abscess formation in a murine thigh infection model of S. aureus. The possibility to track immune cells in vivo by this modality offers new opportunities to investigate host immune response, the efficacy of antibacterial therapies and the influence of virulence factors for pathogenesis.
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DOI：
10.1002/wnan.35
发表时间：
2009-09
影响因子：
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