AUXIN RESPONSE FACTORS 6 and 17 control the flag leaf angle in rice by regulating secondary cell wall biosynthesis of lamina joints.
AUXIN RESPONSE FACTORS 6 and 17 control the flag leaf angle in rice by regulating secondary cell wall biosynthesis of lamina joints.
复制标题
生长素反应因子6和17通过调节叶片接合处次生细胞壁的生物合成来控制水稻旗叶角度。
DOI：
10.1093/plcell/koab175
复制
发表时间：
2021-09-24
期刊：
影响因子：
--
通讯作者：
Zhang D
中科院分区：
文献类型：
--
作者：
Huang G;Hu H;van de Meene A;Zhang J;Dong L;Zheng S;Zhang F;Betts NS;Liang W;Bennett MJ;Persson S;Zhang D
剑叶角度影响密植植物的光合作用能力，因此是作物结构和产量的重要农艺育种性状。激素生长素在调节这一特性中起着关键作用，但其潜在的分子和细胞机制仍不清楚。在这里，我们报道了两个水稻生长素响应因子(ARF)，OsARF6和OsARF17，它们在叶片关节组织中高表达，控制着旗叶角度对生长素的响应。功能丧失的双osarf6 osarf17突变体在密植条件下表现为叶片联合厚壁细胞(SCS)次生细胞壁水平降低，导致旗叶角度增大，籽粒产量下降。力学测量表明，突变体的叶片关节组织太弱，不能支撑旗叶的重量，与水稻增加叶角1(Ila1)突变体的表型相似。我们证明了OsARF6和OsARF17分别独立和协同地直接与ILA1启动子结合以激活其表达。此外，生长素诱导的ILA1表达依赖于OsARF6和OsARF17。总而言之，我们的研究揭示了一种将生长素信号与次生细胞壁成分相结合来确定旗叶角度的机制，为水稻和潜在的其他谷物提供了这一关键性状的育种目标。两个生长素反应基因影响次生细胞壁的生物合成和叶片关节的强度，从而有助于旗叶角度的调整。
Flag leaf angle impacts the photosynthetic capacity of densely grown plants and is thus an important agronomic breeding trait for crop architecture and yield. The hormone auxin plays a key role in regulating this trait, yet the underlying molecular and cellular mechanisms remain unclear. Here, we report that two rice (Oryza sativa) auxin response factors (ARFs), OsARF6 and OsARF17, which are highly expressed in lamina joint tissues, control flag leaf angle in response to auxin. Loss-of-function double osarf6 osarf17 mutants displayed reduced secondary cell wall levels of lamina joint sclerenchymatous cells (Scs), resulting in an exaggerated flag leaf angle and decreased grain yield under dense planting conditions. Mechanical measurements indicated that the mutant lamina joint tissues were too weak to support the weight of the flag leaf blade, resembling the phenotype of the rice increased leaf angle1 (ila1) mutant. We demonstrate that OsARF6 and OsARF17 directly bind to the ILA1 promoter independently and synergistically to activate its expression. In addition, auxin-induced ILA1 expression was dependent on OsARF6 and OsARF17. Collectively, our study reveals a mechanism that integrates auxin signaling with the secondary cell wall composition to determine flag leaf angle, providing breeding targets in rice, and potentially other cereals, for this key trait. Two auxin response genes influence the secondary cell wall biosynthesis and the strength of lamina joints, thereby contributing to the adjustment of flag leaf angles.
登录
查看更多内容
通过X射线μ计算的断层扫描从土壤中恢复完整的植物根系体系。
影响因子：
5.1
作者：
Mairhofer S;Zappala S;Tracy S;Sturrock C;Bennett MJ;Mooney SJ;Pridmore TP
通讯作者：
Pridmore TP
OsmiR167a—靶向生长素反应因子通过微调水稻中生长素的分布来调节分蘖角度
影响因子：
13.8
作者：
Li Y;Li J;Chen Z;Wei Y;Qi Y;Wu C
通讯作者：
Wu C
影响因子：
4.5
作者：
Dong H;Zhao H;Li S;Han Z;Hu G;Liu C;Yang G;Wang G;Xie W;Xing Y
通讯作者：
Xing Y
影响因子：
7.2
作者：
Hong, Z;Ueguchi-Tanaka, M;Matsuoka, M
通讯作者：
Matsuoka, M
影响因子：
5.1
作者：
Haring M;Offermann S;Danker T;Horst I;Peterhansel C;Stam M
通讯作者：
Stam M