Small functional groups for controlled differentiation of hydrogel-encapsulated human mesenchymal stem cells.
Small functional groups for controlled differentiation of hydrogel-encapsulated human mesenchymal stem cells.
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作者：
细胞-基质相互作用在再生、发育和疾病中发挥着关键作用。这里介绍的工作表明，通过使用束缚的小分子化学官能团控制其 3D 环境，可以诱导封装的人间充质干细胞 (hMSC) 分化成骨和脂肪形成途径。使用足够低浓度的系链分子形成水凝胶以维持恒定的物理特性，将 hMSC 封装在 3D 中可防止细胞形态的变化，并且 hMSC 在正常生长培养基中显示出分化，表明小分子功能团诱导分化。据我们所知，这是第一个例子，其中合成基质纯粹通过与水凝胶材料上的小分子化学官能团的相互作用来控制多个 hMSC 谱系的诱导。利用简单化学来控制复杂生物过程的策略将特别有效，因为它们可以使治疗材料的生产更简单、更便宜且更容易控制。
Cell-matrix interactions play critical roles in regeneration, development, and disease. The work presented here demonstrates that encapsulated human mesenchymal stem cells (hMSCs) can be induced to differentiate down osteogenic and adipogenic pathways by controlling their 3D environment using tethered small molecule chemical functional groups. Hydrogels were formed using sufficiently low concentrations of tether molecules to maintain constant physical characteristics, encapsulation of hMSCs in 3D prevented changes in cell morphology, and hMSCs were shown to differentiate in normal growth media, indicating that the small-molecule functional groups induced differentiation. To our knowledge, this is the first example where synthetic matrices are shown to control induction of multiple hMSC lineages purely through interactions with small molecule chemical functional groups tethered to the hydrogel material. Strategies utilizing simple chemistry to control complex biological processes would be particularly are powerful as they could make production of therapeutic materials simpler, cheaper, and more easily controlled.
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影响因子：
14
作者：
Mann, BK;Gobin, AS;West, JL
通讯作者：
West, JL
DOI：
10.1109/memb.2003.1256272
发表时间：
2003-09-01
影响因子：
--
作者：
Kuo, CK;Tuan, RS
通讯作者：
Tuan, RS
DOI：
10.1073/pnas.73.5.1674
发表时间：
1976-01-01
影响因子：
11.1
作者：
MAYNE, R;VAIL, MS;MILLER, EJ
通讯作者：
MILLER, EJ
影响因子：
4.9
作者：
Masters, KS;Shah, DN;Anseth, KS
通讯作者：
Anseth, KS
影响因子：
--
作者：
Caplan, AI
通讯作者：
Caplan, AI