Transcellular and paracellular pathways of transepithelial fluid secretion in Malpighian (renal) tubules of the yellow fever mosquito Aedes aegypti.
Transcellular and paracellular pathways of transepithelial fluid secretion in Malpighian (renal) tubules of the yellow fever mosquito Aedes aegypti.
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黄热病蚊子埃及埃及的马皮亚（肾脏）小管中跨细胞流体分泌的跨细胞和细胞细胞途径。
DOI：
10.1111/j.1748-1716.2010.02195.x
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发表时间：
2011-07
期刊：
影响因子：
--
通讯作者：
Piermarini PM
中科院分区：
文献类型：
--
作者：
Beyenbach KW;Piermarini PM
分离的马氏管分泌NaCl和KCl从管周浴到小管腔通过主动运输Na+和K+的主细胞。结果是管腔阳性跨上皮电压。抗衡离子Cl−通过细胞旁途径通过电扩散被动跟随。水通过渗透作用跟随，但水穿过上皮的具体路线尚不清楚。值得注意的是，NaCl、KCl和水的跨上皮分泌是由位于主细胞的顶端刷状缘膜中的H+ V-ATP酶驱动的，而不是典型的Na/K ATP酶。假设的阳离子/H+交换器将Na+和K+从细胞质移动到小管腔。同样值得注意的是，以开关般的速度对细胞旁通透性进行动态调节，其部分介导了蚊子的血餐后利尿。例如，雌蚊为了滋养卵而摄入的血粉会触发激肽利尿肽的释放，从而1）增加细胞旁途径的Cl−传导，2）组装V1和V0复合物以激活附近的H+ V-ATP酶和阳离子/H+交换。因此，跨细胞和细胞旁途径都被刺激，以快速清除蚊子的血液餐中不需要的盐和水。肾小管的星状细胞似乎起着代谢支持作用，输出在刺激运输活动期间产生的HCO 3 −。分隔连接定义了马氏管中细胞旁途径的性质，但负责分隔连接的选择性渗透和屏障功能的蛋白质是未知的。
Isolated Malpighian tubules of the yellow fever mosquito secrete NaCl and KCl from the peritubular bath to the tubule lumen via active transport of Na+ and K+ by principal cells. Lumen-positive transepithelial voltages are the result. The counter-ion Cl− follows passively by electrodiffusion through the paracellular pathway. Water follows by osmosis, but specific routes for water across the epithelium are unknown. Remarkably, the transepithelial secretion of NaCl, KCl and water is driven by a H+ V-ATPase located in the apical brush border membrane of principal cells and not the canonical Na/K ATPase. A hypothetical cation/H+ exchanger moves Na+ and K+ from the cytoplasm to the tubule lumen. Also remarkable is the dynamic regulation of the paracellular permeability with switch-like speed which mediates in part the post-blood-meal diuresis in mosquitoes. For example, the blood meal the female mosquito takes to nourish her eggs triggers the release of kinin diuretic peptides that 1) increases the Cl− conductance of the paracellular pathway, and 2) assembles V1 and V0 complexes to activate the H+ V-ATPase and cation/H+ exchange close by. Thus, transcellular and paracellular pathways are both stimulated to quickly rid the mosquito of the unwanted salts and water of the blood meal. Stellate cells of the tubule appear to serve a metabolic support role, exporting the HCO3− generated during stimulated transport activity. Septate junctions define the properties of the paracellular pathway in Malpighian tubules, but the proteins responsible for the permselectivity and barrier functions of the septate junction are unknown.
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影响因子：
2.8
作者：
Beyenbach, Klaus W.;Baumgart, Sabine;Zhang, Sheng
通讯作者：
Zhang, Sheng
影响因子：
2.2
作者：
Beyenbach, KW;Masia, R
通讯作者：
Masia, R
影响因子：
2.8
作者：
Cabrero, P;Pollock, VP;Dow, JAT
通讯作者：
Dow, JAT
影响因子：
2.2
作者：
Dow, Julian A. T.;Davies, Shireen A.
通讯作者：
Davies, Shireen A.
影响因子：
2.6
作者：
BRADLEY, TJ;STUART, AM;SATIR, P
通讯作者：
SATIR, P