Sexually dimorphic body color is regulated by sex-specific expression of yellow gene in ponerine ant, Diacamma sp.
Sexually dimorphic body color is regulated by sex-specific expression of yellow gene in ponerine ant, Diacamma sp.
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DOI：
10.1371/journal.pone.0092875
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Miura T
中科院分区：
文献类型：
--
作者：
Miyazaki S;Okada Y;Miyakawa H;Tokuda G;Cornette R;Koshikawa S;Maekawa K;Miura T
由于两性之间的生态差异，大多数膜翅目物种表现出明显的两性二态性。由于单倍二倍体遗传系统下的膜翅目基因组在性别之间表现出数量差异，同时保持质量相同，因此认为性表型是通过性别特异性基因的使用来表达的。在本研究中，研究了无蚁后蚂蚁 Diacamma sp. 表现出明显的颜色二态性的两性二态性表达的分子基础。该物种的雌性工蚁呈蓝黑色，而有翅的雄性则呈现黄棕色的体色。最初，对蛹发育过程中色素沉着过程的观察表明，雌性蛹中存在黑色色素沉着，但雄性蛹中不存在，这表明性别特异性黑色素合成是观察到的颜色二态性的原因。因此，亚克隆了参与昆虫黑色素合成的基因（黄色、乌木、棕褐色、苍白和多巴脱羧酶）的五个直向同源物，并使用实时定量 RT-PCR 检查了它们的时空表达模式。在所检查的基因中，在昆虫黑色素合成中发挥作用的黄色基因在蛹期整个身体中雌性的表达水平高于雄性。然后进行黄色RNA干扰以确定基因功能，并产生了与雄性相似的更黄、更亮的体色的雌性。结论是黄色的转录调控是该物种中观察到的两性颜色二态性的原因。
Most hymenopteran species exhibit conspicuous sexual dimorphism due to ecological differences between the sexes. As hymenopteran genomes, under the haplodiploid genetic system, exhibit quantitative differences between sexes while remaining qualitatively identical, sexual phenotypes are assumed to be expressed through sex-specific gene usage. In the present study, the molecular basis for expression of sexual dimorphism in a queenless ant, Diacamma sp., which exhibits a distinct color dimorphism, was examined. Worker females of the species appear bluish-black, while winged males exhibit a yellowish-brown body color. Initially, observations of the pigmentation processes during pupal development revealed that black pigmentation was present in female pupae but not in males, suggesting that sex-specific melanin synthesis was responsible for the observed color dimorphism. Therefore, five orthologs of the genes involved in the insect melanin synthesis (yellow, ebony, tan, pale and dopa decarboxylase) were subcloned and their spatiotemporal expression patterns were examined using real-time quantitative RT-PCR. Of the genes examined, yellow, which plays a role in black melanin synthesis in insects, was expressed at higher levels in females than in males throughout the entire body during the pupal stage. RNA interference of yellow was then carried out in order to determine the gene function, and produced females with a more yellowish, brighter body color similar to that of males. It was concluded that transcriptional regulation of yellow was responsible for the sexual color dimorphism observed in this species.
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作者：
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发表时间：
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DOI：
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发表时间：
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影响因子：
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作者：
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