Effect of epigenetic modification of maspin on extravillous trophoblastic function
Effect of epigenetic modification of maspin on extravillous trophoblastic function
复制标题
maspin表观遗传修饰对绒毛外滋养层功能的影响
DOI：
10.1007/s11596-012-1051-1
复制
发表时间：
2012
影响因子：
--
通讯作者：
Qiao, Fuyuan
中科院分区：
文献类型：
--
作者：
Zhao, Jun;Chen, Ping;Tang, Guiju;Qiao, Fuyuan
本研究探讨了maspin表观遗传修饰对绒毛外滋养细胞功能的影响。采用RT-PCR方法检测正常妊娠和子痫前期孕妇胎盘组织中maspin mRNA的表达。培养人早孕绒毛外滋养层细胞系TEV-1细胞，用CoCl 2（300 μmol/L）诱导化学缺氧，用5-aza（500 nmol/L）诱导去甲基化。RT-PCR检测不同处理后TEV-1细胞maspin mRNA的表达，CCK-8法和Transwell法检测TEV-1细胞的增殖和迁移能力。结果显示，子痫前期患者胎盘组织中maspin mRNA的表达水平明显高于正常妊娠妇女。CoCl 2和5-aza均能上调maspin mRNA的表达，显著抑制TEV-1细胞的增殖和迁移。结论：maspin基因启动子区的表观遗传修饰导致滋养细胞不完全侵袭，为预测和治疗胎盘功能障碍提供了新的途径。
This study investigated the effect of epigenetic modification of maspin on extravillous trophoblastic function. The mRNA expression of maspin in placentae from normotensive and preeclamptic pregnant women was detected by RT-PCR. TEV-1 cells, a human first-trimester extravillous trophoblast cell line, were cultured and treated with CoCl2(300 μmol/L) to induce chemical hypoxia and with 5-aza (500 nmol/L) to induce demethylation. The mRNA expression of maspin in TEV-1 cells subjected to different treatments was determined by RT-PCR, and the proliferative and migratory abilities of TEV-1 cells were assessed by cell counting kit-8 (CCK-8) and Transwell assays. Our results showed that the maspin mRNA expression level in placentae from preeclamptic women was much higher than that from normotensive women. CoCl2or 5-aza could up-regulate the mRNA expression of maspin and significantly suppress the proliferation and migration of TEV-1 cells. It was concluded that the epigenetic modification in promoter region of maspin contributes to incomplete trophoblast invasion, which offers a novel approach for predicting and treating placental dysfunction.
登录
查看更多内容
DOI：
10.1007/s11596-010-0359-y
发表时间：
2010-06
期刊：
Journal of Huazhong University of Science and Technology [Medical Sciences]
影响因子：
--
作者：
Qiong Zhou;Haiyi Liu;F. Qiao;Yuanyuan Wu;Jingjing Xu
通讯作者：
Qiong Zhou;Haiyi Liu;F. Qiao;Yuanyuan Wu;Jingjing Xu
影响因子：
4.7
作者：
Li, HWR;Leung, SW;Wong, YF
通讯作者：
Wong, YF
影响因子：
3.9
作者：
Qiong Zhou;F. Qiao;Chang Zhao;Haiyi Liu
通讯作者：
Qiong Zhou;F. Qiao;Chang Zhao;Haiyi Liu
影响因子：
3.1
作者：
N. Maass;K. Nagasaki;Martin Ziebart;C. Mundhenke;W. Jonat
通讯作者：
N. Maass;K. Nagasaki;Martin Ziebart;C. Mundhenke;W. Jonat
影响因子：
6.2
作者：
Cai, Zhen;Zhou, Yuan;He, Qing-Yu
通讯作者：
He, Qing-Yu