Transcriptome sequencing and metabolite analysis for revealing the blue flower formation in waterlily.
Transcriptome sequencing and metabolite analysis for revealing the blue flower formation in waterlily.
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转录组测序和代谢物分析揭示睡莲蓝色花朵的形成
DOI：
10.1186/s12864-016-3226-9
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发表时间：
2016-11-09
期刊：
影响因子：
4.4
通讯作者：
Wang LS
中科院分区：
文献类型：
--
作者：
Wu Q;Wu J;Li SS;Zhang HJ;Feng CY;Yin DD;Wu RY;Wang LS
BackgroundWaterlily (Nymphaeaspp.), a perennial herbaceous aquatic plant, is divided into two ecological groups: hardy waterlily and tropical waterlily. Although the hardy waterlily has no attractive blue flower cultivar, its adaptability is stronger than tropical waterlily because it can survive a cold winter. Thus, breeding hardy waterlily with real blue flowers has become an important target for breeders. Molecular breeding may be a useful way. However, molecular studies on waterlily are limited due to the lack of sequence data.ResultsIn this study, six cDNA libraries generated from the petals of two different coloring stages of blue tropical waterlily cultivarNymphaea‘King of Siam’ were sequenced using the Illumina HiSeq™ 2500 platform. Each library produced no less than 5.65 Gb clean reads. Subsequently,de novoassembly generated 112,485 unigenes, including 26,206 unigenes annotated to seven public protein databases. Then, 127 unigenes could be identified as putative homologues of color-related genes in other species, including 28 up-regulated and 5 down-regulated unigenes. In petals, 16 flavonoids (4 anthocyanins and 12 flavonols) were detected in different contents during the color development due to the different expression levels of color-related genes, and four flavonols were detected in waterlily for the first time. Furthermore,UA3GTs were selected as the most important candidates involved in the flavonoid metabolic pathway,UA3GTs induced blue petal color formation inNymphaea‘King of Siam’.ConclusionsThis study will improve our understanding of the molecular mechanism of blue flowers in waterlily and provide the basis for molecular breeding of blue hardy waterlily cultivars.
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影响因子：
4.3
作者：
Bing, So Jin;Ha, Danbee;Jee, Youngheun
通讯作者：
Jee, Youngheun
影响因子：
3.8
作者：
Fossen, T;Froystein, NA;Andersen, OM
通讯作者：
Andersen, OM
影响因子：
4.4
作者：
Feng C;Chen M;Xu CJ;Bai L;Yin XR;Li X;Allan AC;Ferguson IB;Chen KS
通讯作者：
Chen KS
转录组测序和代谢物分析揭示飞燕草素代谢对葡萄风信子花色的作用
影响因子：
6.9
作者：
Lou Q;Liu Y;Qi Y;Jiao S;Tian F;Jiang L;Wang Y
通讯作者：
Wang Y
影响因子：
7.2
作者：
Johnson, ET;Ryu, S;Choi, G
通讯作者：
Choi, G