Characterization of drug-protein interactions in blood using high-performance affinity chromatography.
Characterization of drug-protein interactions in blood using high-performance affinity chromatography.
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DOI：
10.1002/jssc.200800640
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发表时间：
2009-03
影响因子：
3.1
通讯作者：
Joseph, K. S.
中科院分区：
文献类型：
--
作者：
Hage, David S.;Jackson, Abby;Sobansky, Matthew R.;Schiel, John E.;Yoo, Michelle J.;Joseph, K. S.
关键词：
药物与血液、血清或血浆中蛋白质的结合是决定药物在体内的活性、分布、排泄速率和毒性的重要过程。高效亲和层析(HPAC)作为研究这些相互作用的一种手段，受到了极大的关注。本文综述了HPAC中用于检查药物-蛋白质结合的各种技术，并讨论了通过这种方法可以获得的信息类型。还将介绍这些技术与传统结合研究方法(如平衡透析法和超滤法)的比较。然后将讨论HPAC与特定血清蛋白和结合剂的用途，包括人血清白蛋白和α1-酸性糖蛋白。从文献中提供了几个例子来说明这种研究的应用。还介绍了这一领域的最新进展，如使用改进的固定化技术、新的数据分析方法、直接处理复杂生物样品的技术以及固定化脂蛋白的工作。将考虑所述方法的相对优势和局限性，并将讨论这些技术在高通量筛选或表征药物-蛋白质结合方面的可能使用。
The binding of drugs with proteins in blood, serum or plasma is an important process in determining the activity, distribution, rate of excretion, and toxicity of drugs in the body. High-performance affinity chromatography (HPAC) has received a great deal of interest as a means for studying these interactions. This review examines the various techniques that have been used in HPAC to examine drug-protein binding and discusses the types of information that can be obtained through this approach. A comparison of these techniques with traditional methods for binding studies (e.g., equilibrium dialysis and ultrafiltration) will also be presented. The use of HPAC with specific serum proteins and binding agents will then be discussed, including human serum albumin and α1-acid glycoprotein. Several examples from the literature are provided to illustrate the applications of such research. Recent developments in this field are also described, such as the use of improved immobilization techniques, new data analysis methods, techniques for working for directly with complex biological samples, and work with immobilized lipoproteins. The relative advantages and limitations of the methods that are described will be considered and the possible use of these techniques in the high-throughput screening or characterization of drug-protein binding will be discussed.
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影响因子：
7.2
作者：
Chen, HQ;Veluthakal, R;Kowluru, A
通讯作者：
Kowluru, A
影响因子：
3.8
作者：
Day, YSN;Myszka, DG
通讯作者：
Myszka, DG
影响因子：
1.7
作者：
DOMENICI, E;BERTUCCI, C;WAINER, IW
通讯作者：
WAINER, IW
DOI：
10.1016/0021-9673(92)80223-h
发表时间：
1992-02-07
期刊：
JOURNAL OF CHROMATOGRAPHY
影响因子：
--
作者：
ANDERSSON, S;THOMPSON, RA;ALLENMARK, SG
通讯作者：
ALLENMARK, SG
DOI：
10.1016/s0378-4347(01)00493-5
发表时间：
2002-02-25
影响因子：
3
作者：
Andrisano, V;Gotti, R;Bertucci, C
通讯作者：
Bertucci, C