A prospective study on linking diarrheagenic E. coli with stunted childhood growth in relation to gut microbiome.
A prospective study on linking diarrheagenic E. coli with stunted childhood growth in relation to gut microbiome.
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DOI：
10.1038/s41598-023-32491-x
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发表时间：
2023-04-26
影响因子：
4.6
通讯作者：
Bokhari, Habib
中科院分区：
文献类型：
--
作者：
Aziz, Israr;Noreen, Zobia;Ijaz, Umer Zeeshan;Gundogdu, Ozan;Hamid, Muhammad Haroon;Muhammad, Nazir;Khan, Abdullah;Bokhari, Habib
发育迟缓是一个新出现的全球性挑战，影响到中低收入国家的5岁以下儿童。尽管发育迟缓的全球患病率如此之高，但对发病机制和相关肠道微生物群的作用知之甚少。本研究的目的是探讨致病性大肠杆菌菌株的关联。大肠杆菌与生长发育迟缓儿童肠道菌群的关系。收集64份5岁以下儿童粪便标本，进行大肠埃希菌的分离和分子鉴定。杆菌然后使用16S核糖体RNA（rRNA）基因测序分析选定的粪便样本（n = 39，包括3个正常对照）的微生物群落分布。此外，在不同的E.大肠杆菌菌株进行了探索。对分离的E.大肠杆菌（n = 64）中，有39.68%属于大肠杆菌的五种致病型之一。大肠杆菌，而其余的是不可分型的。在不同的致病型中，发现EPEC最普遍（52%; n = 13），其次是EAEC（20%; n = 5）、EIEC（12%; n = 3）、EHEC（8%; n = 2）和ETEC 2（8%; n = 2）。系统发育树分析表明，绝大多数菌株属于B2（28.12%）。微生物多样性被证明是显着的和变化时，样品被组织下回收的植物群。此外，基于预测代谢，发现这些菌株的定殖与能量利用途径如地诺嘌呤-2和乙醛酸-通过显著相关。差异分析表明，土方肠志贺菌和肠球菌改变的儿童发育迟缓。
Stunted growth is an emerging global challenge affecting children under the age of 5 years in low- and middle-income countries. Despite such a high global prevalence of stunting, the mechanism of pathogenesis and the role of associated gut microbiota is poorly understood. The present study was designed to investigate the association of pathogenic strains of E. coli with the residential gut microbiota of stunted growth children. A total of 64 stool sample were collected from children aged ≤ 5 years, and were processed for isolation and molecular characterization of diarrheagenic E. coli. Selected stool samples (n = 39 including three normal controls) were then analysed for microbial community profiling using 16S ribosomal RNA (rRNA) gene sequencing. Furthermore, associations between changes in the microbiota in the presence of different E. coli strains was explored. Pathotyping of the isolated E. coli (n = 64) has shown that 39.68% belonged to one of the five pathotypes of E. coli whilst the remaining ones were non-typeable. Amongst the different pathotypes, EPEC was found to be the most prevalent (52%; n = 13), followed by EAEC (20%; n = 5), EIEC (12%; n = 3), EHEC (8%; n = 2) and ETEC 2 (8%; n = 2). Phylogrouping analysis has shown that majority of the strains belonged to B2 (28.12%). Microbial diversity is shown to be significant and varied when the samples are organized under the recovered phylogroups. Moreover, based on predictive metabolism, the colonization of these strains were found to be significantly associated with energy utilization pathways such as Denovoprine-2 and glyoxylate-by. Differential analysis has shown that Escherichia-Shigella and Enterococcus were altered for the children with stunted growth.
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DOI：
10.1093/cid/ciw542
发表时间：
2016-11-01
期刊：
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
影响因子：
--
作者：
Amour C;Gratz J;Mduma E;Svensen E;Rogawski ET;McGrath M;Seidman JC;McCormick BJ;Shrestha S;Samie A;Mahfuz M;Qureshi S;Hotwani A;Babji S;Trigoso DR;Lima AA;Bodhidatta L;Bessong P;Ahmed T;Shakoor S;Kang G;Kosek M;Guerrant RL;Lang D;Gottlieb M;Houpt ER;Platts-Mills JA;Etiology, Risk Factors, and Interactions of Enteric Infections and Malnutrition and the Consequences for Child Health and Development Project (MAL-ED) Network Investigators
通讯作者：
Etiology, Risk Factors, and Interactions of Enteric Infections and Malnutrition and the Consequences for Child Health and Development Project (MAL-ED) Network Investigators
影响因子：
2.8
作者：
Khalil, Uzma;Younus, Mahwish;Bokhari, Habib
通讯作者：
Bokhari, Habib
影响因子：
7
作者：
Lozupone C;Faust K;Raes J;Faith JJ;Frank DN;Zaneveld J;Gordon JI;Knight R
通讯作者：
Knight R
影响因子：
5.7
作者：
Halaji M;Fayyazi A;Rajabnia M;Zare D;Pournajaf A;Ranjbar R
通讯作者：
Ranjbar R
影响因子：
5.2
作者：
Mushtaq, S.;Irfan, S.;Woodford, N.
通讯作者：
Woodford, N.